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MMP-3/10 Antibody (F-10) | SCBT - Santa Cruz Biotechnology
MMP-3/10 Antibody (F-10) recommended for detection of MMP-3 and MMP-10 of mouse, rat and human origin by WB, IP, IF and ELISA

MMP-3/10 Antibody (F-10): sc-374029

MMP-3/10 Antibody (F-10) is rated 4.6 out of 5 by 5.
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Datasheets

    • MMP-3/10 Antibody (F-10) is a mouse monoclonal IgG1 κ MMP-3/10 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 178-477 mapping at the C-terminus of MMP-3 of human origin
    • MMP-3/10 Antibody (F-10) is recommended for detection of MMP-3 and MMP-10 of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with other MMP family members
    • Anti-MMP-3/10 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP-3/10 (F-10): sc-374029.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MMP-3/10 Antibody (F-10) for WB applications. These reagents are now offered in bundles with MMP-3/10 Antibody (F-10) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with MMP-3/10 (F-10): sc-374029 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Great Features It was a gif and it had started to relieve some of the pain but I need moor.
Date published: 2020-04-30
Rated 5 out of 5 by from WB Working great on western blot. 1:1000 dilution on human prostate cancer cells.
Date published: 2019-05-09
Rated 5 out of 5 by from Give clear bands! I got very clear bands, with no interfering or confusing non specific binding.
Date published: 2018-12-27
Rated 5 out of 5 by from Great immunofluorescence staining of methanol Great immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization. - SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Strong band detected by Western blot analysis Strong band detected by Western blot analysis of MMP-3/10 expression in CCD-1064Sk whole cell lysate. - SCBT QC
Date published: 2015-02-01
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