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MMP-2 Antibody (2C1) | SCBT - Santa Cruz Biotechnology
MMP-2 Antibody (2C1) recommended for detection of MMP-2 of mouse, rat and human origin by WB, IP and IF

MMP-2 Antibody (2C1): sc-13594

MMP-2 Antibody (2C1) is rated 4.3 out of 5 by 41.
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Datasheets

    • MMP-2 Antibody (2C1) is a mouse monoclonal IgG1 κ MMP-2 antibody, cited in 172 publications, provided at 200 µg/ml
    • raised against the activated form of MMP-2 of human origin
    • MMP-2 Antibody (2C1) is recommended for detection of MMP-2 of mouse, rat and human origin by WB, IP and IF
    • MMP-2 Antibody (2C1) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP-2 (2C1): sc-13594.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MMP-2 Antibody (2C1) for WB applications. These reagents are now offered in bundles with MMP-2 Antibody (2C1) (see ordering information below).
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Does it work for bovine tissue?

Asked by: Anonymous
Thank you for your question. This antibody has not yet been tested in bovine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. We do have a previous citation in bovine samples which may be helpful for you: PMID: 12844104.
Answered by: Tech Service
Date published: 2019-11-20

We are looking for a MMP-2 antibody that works well in Western blotting . For this we would like to use a 10ug free sample of MMP2 antibody (2C1): sc-13594. I was wondering if we could get some.

Asked by: Shiv
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-26

Hello.. is there a recommended buffer for use of this product with tissue lysates and immunoblotting? We usually use RIPA buffer for protein expression, but was concerned about chelators.

Asked by: Nabilb
Thank you for your question. We do not have any specific buffer recommendation for use with this MMP-2 antibody and have prepared our lysates for testing using our standard RIPA buffer. You may want to refer to some of the numerous product citations using this antibody for Western blotting to see if they have any optimized recommendations.
Answered by: Technical Support
Date published: 2019-01-26

What is the epitope sequence (binding site) of this antibody? Could you tell me a.a. number?

Asked by: neoSong
Thank you for your question. This mouse monoclonal antibody, MMP-2 (2C1): sc-13594, is raised against the activated form of MMP-2 of human origin. The exact epitope for this monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2017-09-14

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for MMP-2 is available conjugated to agarose (sc-13594 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13594 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13594 PE), fluorescein (sc-13594 FITC), Alexa Fluor® 488 (sc-13594 AF488) or Alexa Fluor® 647 (sc-13594 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 3 out of 5 by from 抗体特异性还可以 用着效果还行。带的大小有些不确定,最上一条带在70kd以下,认为是剪切的mmp2,所以下面的条带不确定是什么。
Date published: 2018-03-20
Rated 5 out of 5 by from Great product MMP-2 antibody works well at the concentration of 1:500 with satisfied bands
Date published: 2018-02-08
Rated 5 out of 5 by from western blot This antibody generates a clearsignal with the HepG2 cell lysis (1:200).
Date published: 2018-01-25
Rated 1 out of 5 by from Unsucessful western blot with glioblastoma cells I have used this antibody 1:100-1:200 for western blotting and failled to obtain any band with the expected molecular weight using lysates from human glioblastoma cell lines
Date published: 2017-11-28
Rated 2 out of 5 by from May not be suitable for IF Sample:muscle cryosection Applications: IF 1:100, 1:200, 1:400 Results: Very weak staining. A little bit staining in nuclear. No difference with negetive control.
Date published: 2017-11-22
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from work well 一月前我向贵公司申请了该抗体的试用装,结果非常好,条带清楚明显,位置正确,抗体很不错,可靠有用,值得再次购买
Date published: 2017-10-19
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