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MMP-13 Antibody (C-3) | SCBT - Santa Cruz Biotechnology
MMP-13 Antibody (C-3) recommended for detection of MMP-13 of human origin by WB, IP, IF and ELISA

MMP-13 Antibody (C-3): sc-515284

MMP-13 Antibody (C-3) is rated 4.8 out of 5 by 9.
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Datasheets

    • MMP-13 Antibody (C-3) is a mouse monoclonal IgG1 κ MMP-13 antibody, cited in 29 publications, provided at 200 µg/ml
    • raised against amino acids 242-471 mapping at the C-terminus of MMP-13 of human origin
    • MMP-13 Antibody (C-3) is recommended for detection of MMP-13 of human origin by WB, IP, IF and ELISA
    • Anti-MMP-13 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP-13 (C-3): sc-515284.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MMP-13 Antibody (C-3) for WB applications. These reagents are now offered in bundles with MMP-13 Antibody (C-3) (see ordering information below).
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Which is best for Neuropathy. i am now getting week in my feet and legs

Asked by: christy
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I have a question. Please give me an answer. Can MMP-13 (C-3): sc-515284 monoclonal antibody be used in IHC with rat chondrocyte?

Asked by: tamu1001
Thank you for your inquiry. Unfortunately, MMP-13 Antibody (C-3): sc-515284 is not recommended use in IHC with rat samples. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 12
Date published: 2022-08-21

This antibody can distinguish whether MMP13 is active form or inactive? WB MMP13 band is not bad.

Asked by: finalb
Thank you for your question. MMP-13 Antibody (C-3): sc-515284 is raised against amino acids 242-471 mapping at the C-terminus of MMP-13 of human origin. This epitope is conserved in both the inactive and active forms of MMP-13 so the antibody should be able to recognize both forms. However, we do not have data demonstrating this reactivity at this time.
Answered by: Technical Support
Date published: 2020-10-08

Can MMP-13 (C-3): sc-515284 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MMP-13 (C-3): sc-515284 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 条带清晰,位置准确 四月初申请的santa 的抗体试用装,收到货后用人软骨细胞系做WB,选择抗体推荐最大稀释比例为1:500,条带位置准确清晰,杂带不是很多,很好用!
Date published: 2018-05-08
Rated 5 out of 5 by from Excellent antibody Worked really good in 1:500 dilution - detected a specific band around 55kDa in conditioned medium.
Date published: 2018-04-14
Rated 5 out of 5 by from Excellent antibody for Western blot The antibody was used at a 1:500 dilution and incubated overnight at 4 degrees Celsius. Using mouse brain tissue we were able to detect three discrete bands which appear to correspond with pro-MMP-13 (~60 kDa) and two cleavage fragments (~48 & ~25 kDa). We are very pleased with this antibody.
Date published: 2017-11-08
Rated 4 out of 5 by from Work well in WB analysis This antibody works well in western blot analysis of mouse liver lysate
Date published: 2017-10-02
Rated 5 out of 5 by from excellent antibody I got 10ug free sample use for pig colon cryosections immunofluorescence stain. the result images are very good.
Date published: 2017-09-11
Rated 5 out of 5 by from It is great I used it for WB, I get a light single band at about 54KD. It is a good antibody.
Date published: 2017-09-03
Rated 5 out of 5 by from strong signal This antibody is very specific and has a high titer than 1:1000,very specific and strong signals .
Date published: 2017-07-22
Rated 5 out of 5 by from MMP-13 antibody Our laboratory research on bone metabolism , and we often use this antibody to detect human arthritis tissue,we got specific band at 48kda by WB ,the antibody diluted at 1:800.
Date published: 2017-07-16
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