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MMP-12 Antibody (A-2) | SCBT - Santa Cruz Biotechnology
MMP-12 Antibody (A-2) recommended for detection of MMP-12 of human origin by WB, IP, IF and ELISA

MMP-12 Antibody (A-2): sc-133151

MMP-12 Antibody (A-2) is rated 4.7 out of 5 by 3.
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Datasheets

    • MMP-12 Antibody (A-2) is a mouse monoclonal IgG2b MMP-12 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 171-470 mapping at the C-terminus of MMP-12 of human origin
    • MMP-12 Antibody (A-2) is recommended for detection of MMP-12 of human origin by WB, IP, IF and ELISA; may cross-react with other MMP family members
    • Anti-MMP-12 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MMP-12 (A-2): sc-133151.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MMP-12 Antibody (A-2). This work is in progress.
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What blocking solution should I use with MMP-12 (A-2): sc-133151 monoclonal antibody for Western blot?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from band at 50kd There was a band at 5kD, but I am not sure whether it is the right band.
Date published: 2016-12-14
Rated 5 out of 5 by from Good WB data with human recombinant MMP Good WB data with human recombinant MMP-12. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Publication with WB and IHC applications Publication with WB and IHC applications using human aortic sample. -SCBT Publication Review
Date published: 2015-01-26
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