MLF1 Antibody (A-8)recommended for detection of MLF1 of human origin by WB, IP, IF and ELISA

MLF1 Antibody (A-8): sc-514304

MLF1 Antibody (A-8) is rated 5.0 out of 5 by 1.
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Datasheets

    • MLF1 Antibody (A-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-135 mapping at the N-terminus of MLF1 of human origin
    • recommended for detection of MLF1 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using MLF1 (A-8): sc-514304?

Asked by: Germaine
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great Western blot data of MLF1 expression Great Western blot data of MLF1 expression in NTERA-2 cl.D1, ES-2 and HeLa whole cell lysates and human hippocampus tissue extract . -SCBT QC
Date published: 2013-01-13
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