MKLP-1 Antibody (C-12)recommended for detection of MKLP-1 of human origin by WB, IP, IF and ELISA

MKLP-1 Antibody (C-12): sc-390113

MKLP-1 Antibody (C-12) is rated 3.8 out of 5 by 4.
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Datasheets

    • MKLP-1 Antibody (C-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-110 mapping at the N-terminus of MKLP-1 of human origin
    • MKLP-1 Antibody (C-12) is recommended for detection of MKLP-1 of human origin by WB, IP, IF and ELISA
    • MKLP-1 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MKLP-1 (C-12): sc-390113.
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The MKLP N-19 is has been discontinued. It worked very well in IF, it stained spindle midzone very clearly with almost no background. I wonder if the C-12 monoclonal also work in IF? If yes, can I see any example?

Asked by: GaryCrick
Thank you for your question. sc-390113 MKLP-1 (C-12) is recommended and warrantied for IF use on human samples. Unfortunately I do not have any published images we can provide at this time. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-06

Can MKLP-1 (C-12): sc-390113 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MKLP-1 (C-12): sc-390113 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 良かったです。 western blotでは500倍希釈で問題なく使用できました。 試しに免疫染色を試みましたが、発色は得られませんでした。
Date published: 2017-12-19
Rated 4 out of 5 by from Try both MKLP-1 antibodies Tried both the N24 and the C-12 antibody for MKLP-1 and one does work better although I am not sure which antibody is the more specific.
Date published: 2017-11-17
Rated 1 out of 5 by from Doesn't work in IF I asked for a free sample to try as I previosuly used N-19 which has been discontinued. But when I fixed cell with 4% paraformaldehyde, it does not work in IF.
Date published: 2017-09-19
Rated 5 out of 5 by from Produced positive Western blot data of MKLP Produced positive Western blot data of MKLP-1 expression in K-562, HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-07-16
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