Mitomycin C CAS: 50-07-7
MF: C15H18N4O5
MW: 334.33
A DNA crosslinking agent that inhibits DNA synthesis, induces apoptosis in a variety of cells, and activates caspase.

Mitomycin C (CAS 50-07-7)

Mitomycin C | CAS 50-07-7 is rated 5.0 out of 5 by 4.
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Alternate Names: Mitomycin C also known as Mitocin-C; Ametycine; Mutamycin; Mitomycin C from Streptomyces caespitosus ;7-amino-9-alpha-methoxymitosane azirino(2′,3′:3,4)pyrrolo(1,2-a)indole-4,7-dione,6-amino-8-(((aminocarbonyl)oxy azirino[2′,3′:3,4]pyrrolo[1,2-a]indole-4,7-dione,6-amino-8-[[(aminocarbonyl)ox mitomycine
Application: Mitomycin C is a DNA crosslinking agent that inhibits DNA synthesis, induces apoptosis in a variety of cells, and activates caspase
CAS Number: 50-07-7
Purity: ≥99%
Molecular Weight: 334.33
Molecular Formula: C15H18N4O5
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
DISCOUNT: Bulk orders of Chemicals totaling $1,000 or more receive a 5% discount.
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Mitomycin C is part of a family of azidine natural products. It is a DNA crosslinking and damaging agent, making it a useful antibiotic and antitumor compound. When crosslinking, Mitomycin C displays absolute specificity and high efficiency for the CpG sequence. Mammalian Cells treated with Mitomycin C (0.4 mg/mL for 5 min) can undergo apoptosis through upregulation of Bad, Fas, FasL and phosphorylated p53 along with activation of caspase-3, caspase-8, and caspase-9 activating both the intrinsic and extrinsic pathway for apoptosis.


References

1. Tomasz, M., et al. 1987. Science. 235: 1204-1208. PMID: 3103215

2. Tomasz, M.,et al. 1988. Biochemistry. 27: 3182-3187. PMID: 3134045

3. Tomasz, M. 1995. Chem. Biol. 2: 575-579. PMID: 9383461

4. Merlo, G.R., et al. 1995. J. Cell Biol. 128: 1185-1196. PMID: 7896881

5. Yoon, Y.S. ,et al. 1996. J. Biol. Chem. 271: 9129-9134. PMID: 8621564

6. Seong, G.J., et al. 2005. Invest. Ophthalmol. Vis. Sci. 46: 3545-3552. PMID: 16186332

Usage :
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be aliquoted and stored at -20°C for up to 1 month. Protect from light, moisture sensitive.
Appearance :
Powder
Physical State :
Solid
Derived From :
Streptomyces caespitosus
Solubility :
Soluble in water (0.5 mg/ml), 100% ethanol (warm) (1 mg/ml), DMSO (15 mg/ml), and methanol.
Storage :
Store at -20° C
Melting Point :
>360° C
Boiling Point :
~581.8° C at 760 mmHg (Predicted)
Density :
~1.6 g/cm3 (Predicted)
Refractive Index :
n20D 1.68 (Predicted)
IC50 :
Cytotoxicity against PC3 cells : EC5050 = 0.14 µM (human); Cytotoxicity against A549 cells: EC5050 = 0.43 µM (human); 2780/DOX ovarian cancer cell line: IC50 = 0.2 µM; promyelocytic leukemic (HL-60) cells: IC50 = 0.05 µM (human); SMMC-7721 (Hepatocellular carcinoma cells): IC50 = 2.44 µg/ml (Homo sapiens); P388 (Lymphoma cells): IC50 = 0.01 µg/ml (Mus musculus)
pK Values :
pKa: 3.2, 6.5, 13.27, pKb: 5.08
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
CN0700000
PubChem CID :
Merck Index :
14: 6215
MDL Number :
MFCD00078109
EC Number :
200-008-6
Beilstein Registry :
7231816
SMILES :
CC1=C(C(=O)C2=C(C1=O)N3C[[email protected]]4[[email protected]@H]([[email protected]@]3([[email protected]@H]2COC(=O)N)OC)N4)N

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Certificate of Analysis

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Mitomycin C (CAS 50-07-7)  Product Citations

See how others have used Mitomycin C (CAS 50-07-7). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 75 total

PMID: # 33929593  Förster, A.|Brand, F.|Banan, R.|Hüneburg, R.|Weber, CAM.|Ewert, W.|Kronenberg, J.|Previti, C.|Elyan, N.|Beyer, U.|Martens, H.|Hong, B.|Bräsen, JH.|Erbersdobler, A.|Krauss, JK.|Stangel, M.|Samii, A.|Wolf, S.|Preller, M.|Aretz, S.|Wiese, B.|Hartmann, C.|Weber, RG.| et al. 2021. Acta Neuropathol.

PMID: # 34706254  Martínez-Martínez, E.|Tölle, R.|Donauer, J.|Gretzmeier, C.|Bruckner-Tuderman, L.|Dengjel, J.| et al. 2021. Matrix Biol.

PMID: # 34298817  Ninou, AH.|Lehto, J.|Chioureas, D.|Stigsdotter, H.|Schelzig, K.|Åkerlund, E.|Gudoityte, G.|Joneborg, U.|Carlson, J.|Jonkers, J.|Seashore-Ludlow, B.|Gustafsson, NMS.| et al. 2021. Cancers (Basel). 13:

PMID: # 34909750  Evrard, C.|Faway, E.|De Vuyst, E.|Svensek, O.|De Glas, V.|Bergerat, D.|Salmon, M.|De Backer, O.|Flamion, B.|Le-Buanec, H.|Lambert de Rouvroit, C.|Poumay, Y.| et al. 2021. JID Innov. 1: 100054.

PMID: # 33761606  Russo, R.|Santarcangelo, C.|Badolati, N.|Sommella, E.|De Filippis, A.|Dacrema, M.|Campiglia, P.|Stornaiuolo, M.|Daglia, M.| et al. 2021. Biomed Pharmacother. 137: 111385.

PMID: # 33567288  Calero-Cuenca, FJ.|Osorio, DS.|Carvalho-Marques, S.|Sridhara, SC.|Oliveira, LM.|Jiao, Y.|Diaz, J.|Janota, CS.|Cadot, B.|Gomes, ER.| et al. 2021. Curr Biol.

PMID: # 34360637  Luis-García, ER.|Becerril, C.|Salgado-Aguayo, A.|Aparicio-Trejo, OE.|Romero, Y.|Flores-Soto, E.|Mendoza-Milla, C.|Montaño, M.|Chagoya, V.|Pedraza-Chaverri, J.|El Hafidi, M.|Orozco-Ibarra, M.|Pardo, A.|Selman, M.| et al. 2021. Int J Mol Sci. 22:

PMID: # 33810010  Lehto, J.|Huguet Ninou, A.|Chioureas, D.|Jonkers, J.|Gustafsson, NMS.| et al. 2021. Cancers (Basel). 13:

PMID: # 34597596  Adeyemi, RO.|Willis, NA.|Elia, AEH.|Clairmont, C.|Li, S.|Wu, X.|D'Andrea, AD.|Scully, R.|Elledge, SJ.| et al. 2021. Mol Cell.

PMID: # 31486569  Carvajal, F.|Duran, C.|Aquea, F.| et al. 2020. J Cosmet Dermatol. 19: 1254-1259.

Citations 1 to 10 of 75 total

Does the vial contain sodium chloride? If yes, what ratio? Thanks :-)

Asked by: Anna87S
Thank you for your question. This product is supplied in solid form and does not contain sodium chloride. For further information, please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-12

How long it can be stored at -80 degrees in solution? Is DMSO the best solvent?

Asked by: Wtaotao
Thank you very much for your question. Mitomycin C (CAS 50-07-7) is supplied as solid and we suggest storing this at -20°C. The product is warranted for up to one year starting from the date of shipping. We have not evaluated long-term storage at -80°C for this product and we do not recommend storage at this temperature. Mitomycin C (CAS 50-07-7) is soluble in water (0.5 mg/ml), 100% ethanol (warm) (1 mg/ml), DMSO (15 mg/ml), and methanol.
Answered by: Technical Support Europe
Date published: 2018-07-18

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. The compound, sc-3514, is provided as a deep blue-purple crystalline powder. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-17

What is the storage temperature after the compound is solubilized?

Asked by: two2igm05
Thank you for your question. After re-suspending this compound in solution, you can store it at -20 degrees Celsius for up to a month without degradation. Tightly seal the cap and wrap it in parafilm. Us it within a month of solubilizing it. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-16
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Rated 5 out of 5 by from I used this product for my mesenchymal cells I used this product for my mesenchymal cells to use as a feeder layer in my experiments. It works well and easily reconstituted unlike Mitomycin C I have used from another company. Will definitely order again.
Date published: 2015-10-29
Rated 5 out of 5 by from This works great in my cell culture studies This works great in my cell culture studies and will order again.
Date published: 2015-10-08
Rated 5 out of 5 by from I have used Mitomycin C for my experiments I have used Mitomycin C for my experiments and I am very pleased with the product. I was working on a project to convert Mouse embryonic stem cells to Neurons and Glia and I needed Mitomycin C to inactivate the feeder cells for ES cell cultivation. The use of this product was smooth and it was easy to reconstitute it in Ethanol. In the past, I have used this same product from a different company and had a lot of issues to reconstitute it in Ethanol. The Santa Cruz product was fantastic. I would recommend this product to all my lab members for their future experiments.
Date published: 2015-09-22
Rated 5 out of 5 by from Howlett Howlett, et. al. (PubMed ID 19704162) used Mitomycin C as a DNA cross-linking agent in treating FA patient cells in order to study functional interaction between FANCD2 and PCNA. -SCBT Publication Review
Date published: 2015-07-01
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