MHC class I Antibody (F-3) recommended for detection of MHC class I of human origin by WB, IP, IF, IHC(P) and ELISA

MHC class I Antibody (F-3): sc-55582

MHC class I Antibody (F-3) is rated 4.9 out of 5 by 9.
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Datasheets

    • MHC class I Antibody (F-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 63-362 of MHC class I of human origin
    • MHC class I Antibody (F-3) is recommended for detection of MHC class I of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-MHC class I Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class I (F-3): sc-55582.
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Which HLA-I isoforms does this clone detect?

Asked by: mmd1
Thank you for your question. This antibody was raised against amino acids 63-362 of MHC class I of human origin. The exact epitope has not been mapped, so more details on which forms that it detects are not available.
Answered by: Tech Service
Date published: 2019-03-28

What do you recommend as the best detection reagent for sc-55582?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Good antibody I'm working with exosomes collected from stimulated NK3.3 cells. Strong signal from exosome Western Blot using 1:1000 antibody dilution.
Date published: 2017-10-26
Rated 5 out of 5 by from Excellent choice for IHC and WB MHC class I Antibody (F-3) is featured in recent publications for IHC and WB.
Date published: 2017-01-10
Rated 5 out of 5 by from Excellent antibody Specifically recognize MHC Class I in human breast cancer
Date published: 2017-01-10
Rated 5 out of 5 by from great i used it for C2C12 and there shows at about 46KDa. single band! very good
Date published: 2016-12-21
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-05
Rated 5 out of 5 by from Good publishable data in WB in human Dendritic Good publishable data in WB in human Dendritic cells (DCs). -SCBT Publication Review
Date published: 2015-05-26
Rated 5 out of 5 by from Excellent immunoperoxidase membrane staining Excellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2015-04-23
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