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Methyl-L-NIO (hydrochloride) is a competitive NOS inhibitor that competes with L-arginine for the amino acid binding site. Methyl-L-NIO (hydrochloride) is a more potent inhibitor of NOS1 (nNOS) (Ki = 3.0 μM) than NOS3 (eNOS) (Ki = 10.0 μM) or NOS2 (iNOS) (Ki = 9.5 μM).
1. Babu, B R., et al., 1998. N5-(1-Imino-3-butenyl)-L-ornithine. A neuronal isoform selective mechanism-based inactivator of nitric oxide synthase. The Journal of biological chemistry. 273(15): 8882-9. PMID: 9535869
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