Molecular structure of MES, CAS Number: 4432-31-9
MES (CAS 4432-31-9)
Alternate Names:
2-(N-Morpholino)ethanesulfonic acid hydrate; 4-Morpholineethanesulfonic acid monohydrate
Application:
MES is a zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays
CAS Number:
4432-31-9
Purity:
≥99%
Molecular Weight:
195.24
Molecular Formula:
C6H13NO4S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
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SDS & Certificate of Analysis
MES is a zwitterionic N-substituted aminosulfonic acid known as a Good′s buffer, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.Also available: MES, Sodium Salt (sc-216095) MES hemisodium salt (sc-216093) MES solution (sc-300959) MES monohydrate (sc-255262)
MES (CAS 4432-31-9) References
- Binding properties of a polyclonal antibody directed towards lead complexes. | Anfossi, L., et al. 2003. Ann Chim. 93: 499-512. PMID: 12911143
- Effect of buffer species on the unfolding and the aggregation of humanized IgG. | Kameoka, D., et al. 2007. J Biochem. 142: 383-91. PMID: 17646171
- Buffer interference with protein dynamics: a case study on human liver fatty acid binding protein. | Long, D. and Yang, D. 2009. Biophys J. 96: 1482-8. PMID: 19217864
- Quantitation of folates and their catabolites in blood plasma, erythrocytes, and urine by stable isotope dilution assays. | Mönch, S., et al. 2010. Anal Biochem. 398: 150-60. PMID: 19903444
- A chemometric approach for optimizing protein covalent immobilization on magnetic core-shell nanoparticles in view of an alternative immunoassay. | Teste, B., et al. 2010. Talanta. 81: 1703-10. PMID: 20441961
- Synthesis and characterization of a novel chitosan-N-acetyl-homocysteine thiolactone polymer using MES buffer. | Ferris, C., et al. 2014. Carbohydr Polym. 111: 125-32. PMID: 25037337
- MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. | Kagenishi, T., et al. 2016. Front Plant Sci. 7: 79. PMID: 26925066
- Agarose native gel electrophoresis of proteins. | Li, C. and Arakawa, T. 2019. Int J Biol Macromol. 140: 668-671. PMID: 31401272
- Comparison of Protein Precipitation Ability of Structurally Diverse Procyanidin-Rich Condensed Tannins in Two Buffer Systems. | Zeller, WE., et al. 2020. J Agric Food Chem. 68: 2016-2023. PMID: 31986021
- Supramolecular Atropine Potentiometric Sensor. | Ferreira, C., et al. 2021. Sensors (Basel). 21: PMID: 34502770
- Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer. | Kuhns, ST., et al. 2022. Biotechnol Prog. e3307. PMID: 36282232
- Hydrogen ion buffers for biological research. | Good, NE., et al. 1966. Biochemistry. 5: 467-77. PMID: 5942950
- Ion exchange resins for the purification of monoclonal antibodies from animal cell culture. | Graf, H., et al. 1994. Bioseparation. 4: 7-20. PMID: 7764588
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MES, 25 g | sc-216094B | 25 g | $32.00 | |||
MES, 100 g | sc-216094 | 100 g | $86.00 | |||
MES, 250 g | sc-216094A | 250 g | $180.00 | |||
MES, 1 kg | sc-216094C | 1 kg | $499.00 | |||
MES, 5 kg | sc-216094D | 5 kg | $1975.00 | |||
MES, 10 kg | sc-216094E | 10 kg | $3899.00 |