MeOSuc-AAPV-AMC CAS: 72252-90-5
MF: C31H41N5O9
MW: 627.69
A fluorogenic substrate used to assay elastase activity.

MeOSuc-AAPV-AMC (CAS 72252-90-5)

MeOSuc-AAPV-AMC | CAS 72252-90-5 is rated 5.0 out of 5 by 1.
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Alternate Names: Neutrophil elastase substrate (fluorogenic); N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin; Elastase Substrate V
Application: MeOSuc-AAPV-AMC is a fluorogenic substrate used to assay elastase activity
CAS Number: 72252-90-5
Purity: ≥95%
Molecular Weight: 627.69
Molecular Formula: C31H41N5O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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MeOSuc-AAPV-AMC is a fluorogenic substrate of neutrophil elastase. This compound can be used to assay elastase activity by spectrofluorometrically monitoring elastase-produced hydrolysis products. Assays of human leukocytes and porcine pancreatic elastase is described with use of MeOSuc-AAPV-AMC as a probe.


References

1. Castillo, M.J., et al. 1979. Anal. Biochem. 99: 53-64. PMID: 394626
2. Edwards, K.M., et al. 1999. J. Biol. Chem. 274: 30468-30473. PMID: 10521426
3. Coles, B., et al. 2002. Circ. Res. 91: 375-381. PMID: 12215485
4. Cloutier, S.M., et al. 2004. Eur. J. Biochem. 271: 607-613. PMID: 14728688

Physical State :
Solid
Sequence :
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amino-4-methylcoumarin
Solubility :
Soluble in DMSO (>3.2 mM), ethanol, water, and chloroform (50 mg/ml).
Storage :
Store at -20° C
Boiling Point :
~983.7° C at 760 mmHg (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.57
Fluoresence :
λex 380 nm, λem 460 nm in conditioned medium; λex 355 nm, λem 440 nm in Conditioned medium; λex 375 nm, λem 450 nm in Conditioned medium
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
MDL Number :
MFCD00077135
SMILES :
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(C(C)C)NC(=O)C3CCCN3C(=O)C(C)NC(=O)C(C)NC(=O)CCC(=O)OC

Download SDS (MSDS)

Certificate of Analysis

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MeOSuc-AAPV-AMC (CAS 72252-90-5)  Product Citations

See how others have used MeOSuc-AAPV-AMC (CAS 72252-90-5). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 31478257  2019. J. Leukoc. Biol.

PMID: # 26974397  Habgood, AN.|Tatler, AL.|Porte, J.|Wahl, SM.|Laurent, GJ.|John, AE.|Johnson, SR.|Jenkins, G.| et al. 2016. Lab Invest. 96: 623-31.

PMID: # 25365140  Budnjo, A. et al. 2014. J. Med. Chem. 57: 9396-408.

PMID: # 32971767  Cells.

PMID: # 33322797  Cells.

PMID: # 31216421  FASEB J. 10443-10452.

PMID: # 32209981  Cells.

PMID: # 31422870  Immunity. 443-450.e4.

PMID: # 32745993  EBioMedicine. 102925.

PMID: # 30369266  Autoimmunity. 297-303.

Citations 1 to 10 of 13 total

Hi, I was wondering which substrate is more specific for PR3 enzyme MeOSuc-AAPV-pNA or MeOSuc-AAPV-AMC?

Asked by: AAftab
Thank you for your question. MeOSuc-AAPV-pNA has been found to be a substrate for PR3, we do not have any literature supporting MeOSuc-AAPV-AMC being a highly sensitive substrate for PR3.
Answered by: Technical Support
Date published: 2017-04-05

Any recommendations for solubility in aqueous buffers?

Asked by: chemicalsmg
Thank you for your question. This chemical is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, first dissolve in DMF and then dilute with the aqueous buffer of choice. Solubility is approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-12
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Rated 5 out of 5 by from Various publications cite the use of MeOSuc Various publications cite the use of MeOSuc-AAPV-AMC to assay elastase activity by spectrofluorometrically monitoring elastase-produced hydrolysis products. -SCBT Publication Review
Date published: 2015-06-09
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