Anti-MEL-1A/B-R Antibody (B-8) recommended for detection of MEL-1A-R and MEL-1B-R of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MEL-1A/B-R Antibody (B-8): sc-398788

Anti-MEL-1A/B-R Antibody (B-8) is rated 4.5 out of 5 by 11.
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Datasheets

    • MEL-1A/B-R Antibody (B-8) is a mouse monoclonal IgM κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 161-280 mapping within an internal region of MEL-1A-R of human origin
    • recommended for detection of MEL-1A-R and MEL-1B-R of mouse, rat and human origin by WB, IP, IF and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MEL-1A/B-R (B-8): sc-398788.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for MEL-1A/B-R Antibody (B-8) for WB applications. This reagent is now offered in a bundle with MEL-1A/B-R Antibody (B-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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any blocking peptide available for MEL-1A/B-R? Why not separate antibody for specific MT1 and MT2 receptors?

Asked by: Tavleen
Thank you for your question. Unfortunately there is no blocking peptide available for this antibody as it was not made against a peptide. We would suggest using a negative control using a control IgG. We do not offer separate antibodies for the two receptors currently.
Answered by: Tech Service
Date published: 2017-06-15

Are there any issues with using sc-398788: MEL-1A/B-R (B-8) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-398788 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from great antibody This was tested for hRMECs. This work incredibly well with high specificity and very low background.Recommend use dilution 1:200.
Date published: 2021-01-28
Rated 1 out of 5 by from No success. We tested this antibody with pancreas and brain from mouse and rat. Unfortunately we did not detect the protein.
Date published: 2019-09-19
Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat protein,the band is quite strong
Date published: 2019-02-25
Rated 5 out of 5 by from Great antibody We got obtained specific band is very clear and powerful signal.Knowledge of the sensitivity and specificity of MEL-1A/B-R Antibody leads to their more rational selection.
Date published: 2017-10-09
Rated 5 out of 5 by from worked well This was tested for mouse spleen sections. This work incredibly well with high specificity and very low background.Recommend use dilution 1:250.
Date published: 2017-08-12
Rated 4 out of 5 by from Happy monoclonal antibody We used MEL-1A-R monoclonal antibody in the sample mouse cells, when WB dilution 1:500 most obvious effect, we are very happy.
Date published: 2017-05-18
Rated 5 out of 5 by from Strong single bands Strong single bands seen in Western Blot with no background or additional bands.
Date published: 2017-03-05
Rated 5 out of 5 by from Wonderful This antibody gives nice clean bands on WB, the best monoclonal antibody we've found.
Date published: 2017-02-12
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