Anti-MEK-3 Antibody (B-5) recommended for detection of MEK-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-MEK-3 Antibody (B-5): sc-271779

Anti-MEK-3 Antibody (B-5) is rated 4.0 out of 5 by 6.
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Datasheets

    • Anti-MEK-3 Antibody (B-5) is a mouse monoclonal IgG3 κ MEK-3 antibody, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 32-61 at the N-terminus of MEK-3 of human origin
    • recommended for detection of MEK-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MEK-3 (B-5): sc-271779.
    • m-IgG3 BP-HRP is the preferred secondary detection reagent for MEK-3 Antibody (B-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with MEK-3 Antibody (B-5) (see ordering information below).
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What application is the blocking peptide sc-271779 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from Reviews misleading- pertain to OLD Ab This antibody has been tested in four cell types, two tissues. They sent us two separate lots. We published over 20 papers with the previous version, made in a different host, and used archived lysates to test this antibody No reactivity to MEK-3 in mice.
Date published: 2019-09-16
Rated 4 out of 5 by from Good for IF This MEK-3 Antibody (B-5), sc-271779, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Potent and fairly specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~40 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~52 kDa medium cross-reactive protein was detectable in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Published western blot data using human T Published western blot data using human T-lymphoma Jurkat cell line. -SCBT Publication Review
Date published: 2015-02-08
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-02-05
Rated 5 out of 5 by from Strong band at expected MW in human transfected Strong band at expected MW in human transfected 293 lysates. -SCBT QC
Date published: 2015-01-16
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