Anti-MEK-2 Antibody (96) recommended for detection of MEK-2 of mouse, rat, human and canine origin by WB, IP and IF

Anti-MEK-2 Antibody (96): sc-136261

Anti-MEK-2 Antibody (96) is rated 4.0 out of 5 by 3.
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Datasheets

    • MEK-2 Antibody (96) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 1-110 of MEK-2 of rat origin
    • recommended for detection of MEK-2 of mouse, rat, human and canine origin by WB, IP and IF
    • See MEK-2 (A-1): sc-13159 for MEK-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MEK-2 Antibody (96). This work is in progress.
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I am using MEK-2 (96): sc-136261 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced good immunofluorescence cytoplasmic Produced good immunofluorescence cytoplasmic staining in human fibroblast cells. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Nice band achieved in WB with RSV Nice band achieved in WB with RSV-3T3 whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Positive signal obtained in Western with human MEK Positive signal obtained in Western with human MEK-2 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-04-11
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