Anti-MEK-2 Antibody (8E8) recommended for detection of MEK-2 of mouse, rat, human and canine origin by WB and IP

Anti-MEK-2 Antibody (8E8): sc-81473

Anti-MEK-2 Antibody (8E8) is rated 4.0 out of 5 by 2.
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Datasheets

    • MEK-2 Antibody (8E8) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against N-terminus of MEK-2 of human origin
    • recommended for detection of MEK-2 of mouse, rat, human and canine origin by WB and IP
    • See MEK-2 (A-1): sc-13159 for MEK-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MEK-2 Antibody (8E8). This work is in progress.
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I am using MEK-2 (8E8): sc-81473 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Positive western results when 8E8 tested with non Positive western results when 8E8 tested with non-transfected 293T, human MEK-2 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Strong band clearly observed in western blot Strong band clearly observed in western blot analysis of MEK-2 expression in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2013-10-14
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