Mdr-1 Antibody (G-1)recommended for detection of Mdr-1 of human origin by WB, IP, IF, IHC(P) and ELISA

Mdr-1 Antibody (G-1): sc-13131

Mdr-1 Antibody (G-1) is rated 5.0 out of 5 by 10.
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Datasheets

    • Mdr-1 Antibody (G-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1040-1280 of Mdr -1 of human origin
    • Mdr-1 Antibody (G-1) is recommended for detection of Mdr-1 of human origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with Mdr-3
    • Mdr-1 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mdr-1 (G-1): sc-13131.
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Can Mdr-1 (G-1): sc-13131 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Mdr-1 (G-1): sc-13131 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-11

What secondary is best to use with Mdr-1 (G-1): sc-13131 monoclonal antibody?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Mdr-1 (G-1): sc-13131, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 5 out of 5 by from Strong signals in Western and specific staining in Strong signals in Western and specific staining in IHC
Date published: 2018-05-19
Rated 5 out of 5 by from Great Western blot Testing different specificity of Mdr-1 expression, to detect the effect is very good
Date published: 2017-08-17
Rated 5 out of 5 by from Obvious effect Mdr-1 antibody worked well in the human samples, the antibody shows hight express.
Date published: 2017-05-09
Rated 5 out of 5 by from Praise When work in Mdr-1 Antibody in imprinting feel good, also can be used in IP and IHC.
Date published: 2017-04-26
Rated 5 out of 5 by from Wow; superb data Just a great antibody for multiple applications!!!
Date published: 2017-01-29
Rated 5 out of 5 by from Great for IHC(P) The Mdr-1 Antibody (G-1): sc-13131 provides excellent staining pattern for IHC applications when using formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited over 40 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Working very well in Western Blot Working very well in Western Blot. DLD-1 colon cancer cell extract was used as a positive control.
Date published: 2015-03-24
Rated 5 out of 5 by from Very nice immunostaining with this antibody Very nice immunostaining with this antibody -SCBT QC
Date published: 2015-02-16
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