Anti-MCT2 Antibody (D-5) recommended for detection of MCT2 of mouse origin by WB, IP, IF, IHC(P) and ELISA

Anti-MCT2 Antibody (D-5): sc-166925

Anti-MCT2 Antibody (D-5) is rated 4.7 out of 5 by 15.
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Datasheets

    • Anti-MCT2 Antibody (D-5) is a mouse monoclonal IgG2b κ MCT2 antibody, cited in 15 publications, provided at 200 µg/ml
    • raised against amino acids 391-480 mapping at the C-terminus of MCT2 of mouse origin
    • MCT2 Antibody (D-5) is recommended for detection of MCT2 of mouse origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-MCT2 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCT2 (D-5): sc-166925.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCT2 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with MCT2 Antibody (D-5) (see ordering information below).
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Is this antibody suitable for immunofluorescence on frozen brain slices?

Asked by: RobertaT84
Thank you for your question. This antibody is recommended for IF, or immunofluorescence on fresh or frozen tissues or cells fixed in acetone or methanol. We do not yet have published data on frozen brain tissue, but we would still warranty the antibody for that application. We do have published data in formalin-fixed paraffin-embedded tissue sections of mouse kidney, testes and stomach.
Answered by: Technical Support
Date published: 2019-03-21

I am using MCT2 (D-5): sc-166925 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for IF This antibody works well in my immunostaining experiment with mouse samples.
Date published: 2018-06-05
Rated 5 out of 5 by from Very specific I got free sample of this last month.It worked really well. I am going to buy full size sample soon.
Date published: 2018-04-20
Rated 1 out of 5 by from Kein Ergebnis im WB hat beim durchgeführten Westernblot kein Signal gebracht
Date published: 2018-04-03
Rated 5 out of 5 by from Great antibody Used for Western blot in mouse cell lines. Works great!
Date published: 2017-12-22
Rated 5 out of 5 by from Sehr gute Ergebisse im Western Blot Bei der Probegröße hatten wir gute Ergebnisse im Western Blot bei einer Verdünnung von 1:100.
Date published: 2017-11-22
Rated 5 out of 5 by from excellent We made an experiment with MCT2 Antibody antibody and found it very useful. I have a clear band and the binding is both strong and specific.
Date published: 2017-10-24
Rated 5 out of 5 by from excellent We made an experiment with MCT2 Antibody and found it very useful. The background is clear, simple operation, short cycle, which has been widely applied.
Date published: 2017-10-23
Rated 4 out of 5 by from WB got a strong signal. We used this antibody in Western blotting with 1:200 dilution. WB got a strong signal, we are very happy about the success of the experiment
Date published: 2017-05-18
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