MCM7 Antibody (SPM379)recommended for detection of MCM7 of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)

MCM7 Antibody (SPM379): sc-56429

MCM7 Antibody (SPM379) is rated 4.5 out of 5 by 2.
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Datasheets

    • MCM7 Antibody (SPM379) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant hCDC47 of human origin
    • recommended for detection of MCM7 of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See MCM7 (141.2): sc-9966 for MCM7 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using MCM7 (SPM379): sc-56429?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from WB: tested with HL WB: tested with HL-60 and A-431 nuclear extracts and HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Strong Strong, clean bands in western blot using COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-08-24
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