Anti-MCM7 Antibody (G-7) recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MCM7 Antibody (G-7): sc-46687

Anti-MCM7 Antibody (G-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • MCM7 Antibody (G-7) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of MCM7 of human origin
    • recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See MCM7 (141.2): sc-9966 for MCM7 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MCM7 Antibody (G-7) for WB applications. These reagents are now offered in bundles with MCM7 Antibody (G-7) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with MCM7 (G-7): sc-46687 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclear Produced exceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Western blot and CoIP publication using breast Western blot and CoIP publication using breast (MCF10A, MCF10AT, and MCF10AC1a cells) -SCBT Publication Review
Date published: 2015-03-24
Rated 5 out of 5 by from Positive signal via immunofluorescence staining Positive signal via immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Western blot publication using human samples Western blot publication using human samples HeLa S3 -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Publication using mouse the luminal content Publication using mouse the luminal content of the caudal epididymides and vas deferens -SCBT Publication Review
Date published: 2015-02-16
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