Anti-MCM7 Antibody (DCS-141) recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-MCM7 Antibody (DCS-141): sc-65469

Anti-MCM7 Antibody (DCS-141) is rated 5.0 out of 5 by 8.
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Datasheets

    • MCM7 Antibody (DCS-141) is a mouse monoclonal IgG1 κ, cited in 19 publications, provided at 200 µg/ml
    • raised against full length MCM7 of human origin
    • recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCM7 (DCS-141): sc-65469.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MCM7 Antibody (DCS-141) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM7 Antibody (DCS-141) (see ordering information below).
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I am using MCM7 (DCS-141): sc-65469 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent for WB in both human and chicken cells The antibody was used at the dilution of 1:200. The band was specific and the signal was strong!
Date published: 2021-02-23
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Excellent for WB Strong band with 1:1,000 dilution by chemiluminescence detection (mouse tissue samples)
Date published: 2017-08-04
Rated 5 out of 5 by from Successfully immunoprecipitated MCM7 from U Successfully immunoprecipitated MCM7 from U-2-OS and transformed ATM-deficient cells and verified via western blot. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear staining Exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from WB: works well with RECs WB: works well with RECs. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Good antibody for western blot; used with MCF Good antibody for western blot; used with MCF-7, ZR-75, BT-549, MDA MB-468, MDA MB-453, MDA MB-231, T47D, SKBR-3, PMC-42DU-145 and PC-3, LNCaP cells. -SCBT Publication Review
Date published: 2015-01-27
Rated 5 out of 5 by from Positive results in COLO 320DM Positive results in COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-02
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