Anti-MCM7 Antibody (0.N.194) recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-MCM7 Antibody (0.N.194): sc-71550

Anti-MCM7 Antibody (0.N.194) is rated 4.0 out of 5 by 2.
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Datasheets

    • MCM7 Antibody (0.N.194) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • raised against full length MCM7 of human origin
    • recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See MCM7 (141.2): sc-9966 for MCM7 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM7 Antibody (0.N.194) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM7 Antibody (0.N.194) (see ordering information below).
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What blocking solution should I use with MCM7 (0.N.194): sc-71550 monoclonal antibody for Western blot?

Asked by: Randy McDonald
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Robust bands observed in western blot analysis Robust bands observed in western blot analysis of MCM7 expression in non-transfected 293T, human MCM7 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 4 out of 5 by from Slightly faint bands present in western blot Slightly faint bands present in western blot using COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-08-05
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