MCM7 Antibody (0.N.194)recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and IHC(P)

MCM7 Antibody (0.N.194): sc-71550

MCM7 Antibody (0.N.194) is rated 4.0 out of 5 by 2.
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Datasheets

    • MCM7 Antibody (0.N.194) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length MCM7 of human origin
    • recommended for detection of MCM7 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See MCM7 (141.2): sc-9966 for MCM7 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What blocking solution should I use with MCM7 (0.N.194): sc-71550 monoclonal antibody for Western blot?

Asked by: Randy McDonald
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Robust bands observed in western blot analysis Robust bands observed in western blot analysis of MCM7 expression in non-transfected 293T, human MCM7 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 4 out of 5 by from Slightly faint bands present in western blot Slightly faint bands present in western blot using COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-08-05
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