Anti-MCM6 Antibody (H-8) recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-MCM6 Antibody (H-8): sc-393618

Anti-MCM6 Antibody (H-8) is rated 4.8 out of 5 by 13.
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Datasheets

    • Anti-MCM6 Antibody (H-8) is a mouse monoclonal IgG2a κ MCM6 antibody, cited in 12 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 792-821 at the C-terminus of MCM6 of human origin
    • Anti-MCM6 Antibody (H-8) is recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
    • Anti-MCM6 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCM6 (H-8): sc-393618.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MCM6 Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM6 Antibody (H-8) (see ordering information below).
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What application is the blocking peptide sc-393618 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent antibody - good strength Antibody sensitivity is extremely strong. All expected expression patterns were observed in initial tests.
Date published: 2021-07-28
Rated 5 out of 5 by from Perfect for WB in human and chicken cells Antibody was used at the dilution of 1:200. The band was specific and the signal was strong.
Date published: 2021-02-23
Rated 5 out of 5 by from Great antibody! Great antibody for western blot. Clear target band!
Date published: 2019-04-22
Rated 5 out of 5 by from good western results 基本 没 有 杂带,牛奶封闭,一抗过夜,二抗牛奶室温两小时,30ug细胞蛋白,跑出条带如下图所示
Date published: 2018-03-11
Rated 3 out of 5 by from Good for western but seems not the best for IF I tried this antibody with both western and IF. Western showed a weak nonspecific band though the main band was strong and clear. As for IF, the staining looked not that neat compared to other MCM antibodies. If I perform IF with MCM, I prefer MCM7 antibody (sc-65469).
Date published: 2017-11-21
Rated 5 out of 5 by from a fantastic antibody for WB This antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Great for WB Very specific band at a dilution 1:1,000 for mouse tissue lysates
Date published: 2017-08-04
Rated 5 out of 5 by from Great antibody MCM6 Antibody was used in WB with 1:1000 dilution, the band is strong and specific.
Date published: 2017-04-08
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