Anti-MCM6 Antibody (D-12) recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MCM6 Antibody (D-12): sc-55577

Anti-MCM6 Antibody (D-12) is rated 5.0 out of 5 by 3.
  • y_2022, m_1, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_55577, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 109ms
  • REVIEWS, PRODUCT
loading
5
3
4
0
3
0
2
0
1
0

Datasheets

    • MCM6 Antibody (D-12) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of MCM6 of human origin
    • recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM6 Antibody (D-12) for WB applications. These reagents are now offered in bundles with MCM6 Antibody (D-12) (see ordering information below).
loading

What is the recommended fixation method for immunofluorescence staining with MCM6 (D-12): sc-55577 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2022, m_1, d_19, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_55577, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Clean bands in western blot analysis of MCM6 Clean bands in western blot analysis of MCM6 expression in HeLa, ALL-SIL, MM-142, NIH/3T3, KNRK, K-562 and Jurkat (G) whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Published results of CoIP/WB using Mcm transfected Published results of CoIP/WB using Mcm transfected CHO cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-01
  • y_2022, m_1, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_55577, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT