MCM6 Antibody (D-12) recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF and ELISA

MCM6 Antibody (D-12): sc-55577

MCM6 Antibody (D-12) is rated 5.0 out of 5 by 3.
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Datasheets

    • MCM6 Antibody (D-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of MCM6 of human origin
    • recommended for detection of MCM6 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What is the recommended fixation method for immunofluorescence staining with MCM6 (D-12): sc-55577 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Clean bands in western blot analysis of MCM6 Clean bands in western blot analysis of MCM6 expression in HeLa, ALL-SIL, MM-142, NIH/3T3, KNRK, K-562 and Jurkat (G) whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Published results of CoIP/WB using Mcm transfected Published results of CoIP/WB using Mcm transfected CHO cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-01
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