Mcm4 Antibody (C-12)recommended for detection of Mcm4 of y origin by WB, IP, IF and ELISA

Mcm4 Antibody (C-12): sc-166036

Mcm4 Antibody (C-12) is rated 4.5 out of 5 by 2.
  • y_2020, m_1, d_16, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_166036, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 125ms
  • REVIEWS, PRODUCT
5
1
4
1
3
0
2
0
1
0

Datasheets

    • Mcm4 Antibody (C-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Mcm4 of Saccharomyces cerevisiae origin
    • Mcm4 Antibody (C-12) is recommended for detection of Mcm4 of y origin by WB, IP, IF and ELISA
    • Mcm4 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mcm4 (C-12): sc-166036.
Submit a review for this product and receive 15 CruzCredits

We want to use Rad53 (sc-74429) and MCM4 (sc-166036). Do you give samples to test these antibodies? 

Asked by: Sathyan
Thank you for your question. We do offer a samples of sc-74427 and sc-166036. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-07

What blocking solution should I use with Mcm4 (C-12): sc-166036 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
  • y_2020, m_1, d_16, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_166036, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 157ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from The antibody worked! I got a positive result with the dilution factor 1:500 on a PVDF membrane. The band was relatively strong, so I might want to try to increase the dilution factor 1:1000 in next experiment.
Date published: 2017-03-19
Rated 5 out of 5 by from Positive results in Western blot analysis Positive results in Western blot analysis of yeast recombinant Mcm4 fusion protein. -SCBT QC
Date published: 2013-08-11
  • y_2020, m_1, d_16, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_166036, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.