MCM4 Antibody (C-10)recommended for detection of MCM4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

MCM4 Antibody (C-10): sc-48407

MCM4 Antibody (C-10) is rated 5.0 out of 5 by 4.
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Datasheets

    • MCM4 Antibody (C-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of MCM4 of human origin
    • recommended for detection of MCM4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using MCM4 (C-10): sc-48407 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Signal via immunoperoxidase staining of formalin Signal via immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of cells in germinal and non-germinal centers. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Great immunofluorescence nuclear staining Great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Signal via western blot analysis of MCM4 Signal via western blot analysis of MCM4 expression in HeLa, NIH/3T3 and KNRK nuclear extracts. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Publication using Mcm transfected CHO cells Publication using Mcm transfected CHO cells in CoIP and WB applications -SCBT Publication Review
Date published: 2014-08-07
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