Anti-MCM3 Antibody (E-8) recommended for detection of MCM3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-MCM3 Antibody (E-8): sc-390480

Anti-MCM3 Antibody (E-8) is rated 4.6 out of 5 by 15.
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Datasheets

    • Anti-MCM3 Antibody (E-8) is a mouse monoclonal IgG1 κ MCM3 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 1-215 mapping at the N-terminus of MCM3 of human origin
    • Anti-MCM3 Antibody (E-8) is recommended for detection of MCM3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
    • Anti-MCM3 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCM3 (E-8): sc-390480.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM3 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM3 Antibody (E-8) (see ordering information below).
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I am using MCM3 (E-8): sc-390480 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very specific band at about 115kDa I used Goat anti MCM3 previously, sc9850, but it's finished. By changing this new one, I can obtain the exact specific band at about 115kDa against mouse cell lysates but haven't tried in tissue proteins. I dilute at 1:1000 in 5%milk/TBST.
Date published: 2021-06-29
Rated 5 out of 5 by from Great antibody We use this antibody 1:500 for western blotting detection of MCM3. Higly specific.
Date published: 2019-08-23
Rated 3 out of 5 by from It works in some samples It worked well in our samples. But somehow it didn't work in some of our samples compared to other company.
Date published: 2018-06-29
Rated 4 out of 5 by from This antibody performed well This antibody performed well. But we found two bands in our human sample, one is around 75 kDa and another one is around 115 kDa where the datasheet points out.
Date published: 2018-04-19
Rated 5 out of 5 by from 抗体可以的 very 基本没有杂带,牛奶封闭,一抗过夜,二抗牛奶室温两小时,30ug细胞蛋白,跑出条带如下图所示
Date published: 2018-03-11
Rated 4 out of 5 by from Clean Western data & reasonable IF data I used this antibody in my western blotting (1 to 200 dilution) and IF (1 to 100 dilution). In western, I used whole cell extracts from hTERT-RPE1 cells and I could get a nice clean single band. As for IF, this MCM3 antibody showed neat data as good as MCM7 antibody (sc-56324), however, signal strength looked weaker than anti-MCM7. If I'd see any MCMs, I choose MCM7 antibody though if I'd specifically see MCM3, I'll consider using this antibody.
Date published: 2017-11-10
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from One of the best antibodies for WB One of the best antibodies for WB that I have ever used. Single band at about 120 kd. Now we are trying IP experiments.
Date published: 2017-08-22
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