Mcm2 Antibody (C-3)recommended for detection of Mcm2 of S. cerevisiae origin by WB, IP, IF and ELISA

Mcm2 Antibody (C-3): sc-515723

Mcm2 Antibody (C-3) is rated 2.0 out of 5 by 2.
  • y_2020, m_1, d_18, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_515723, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 246ms
  • REVIEWS, PRODUCT
5
0
4
0
3
0
2
2
1
0

Datasheets

    • Mcm2 Antibody (C-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 11-34 near the N-terminus of Mcm2 of Saccharomyces cerevisiae origin
    • Mcm2 Antibody (C-3) is recommended for detection of Mcm2 of S. cerevisiae origin by WB, IP, IF and ELISA
    • Mcm2 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Mcm2 (C-3): sc-515723.
Submit a review for this product and receive 15 CruzCredits

What blocking solution should I use with Mcm2 (C-3): sc-515723 monoclonal antibody for Western blot?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
  • y_2020, m_1, d_18, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_515723, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 129ms
  • QUESTIONS, PRODUCT
Rated 2 out of 5 by from 跑出来带比较浅,跟说明书不太一样 试用装跑出来带比较浅,跟说明书不太一样,就一个条带 30ug蛋白,5%脱脂牛奶封闭,一抗过夜孵育,二抗牛奶室温两个小时
Date published: 2018-03-11
Rated 2 out of 5 by from not a great antibody for WB This antibody generates a very weak (barely detectable) signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). Perhaps loading more extract would be helpful
Date published: 2017-09-18
  • y_2020, m_1, d_18, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_515723, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.