Anti-MBP-probe Antibody (TVG MBP 3) recommended for detection of MBP fusion proteins by WB and IP

Anti-MBP-probe Antibody (TVG MBP 3): sc-53367

Anti-MBP-probe Antibody (TVG MBP 3) is rated 4.0 out of 5 by 1.
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Datasheets

    • MBP-probe Antibody (TVG MBP 3) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against MOS maltose binding protein (MBP) fusion protein
    • recommended for detection of MBP fusion proteins by WB and IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MBP-probe Antibody (TVG MBP 3). This work is in progress.
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I am using MBP-probe (TVG MBP 3): sc-53367 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good band seen in WB using MBP Good band seen in WB using MBP-tagged fusion protein. -SCBT QC
Date published: 2014-10-31
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