Anti-MASP-2 Antibody (B-10) recommended for detection of MASP-2 A chain of human origin by WB, IP, IF and ELISA

Anti-MASP-2 Antibody (B-10): sc-390200

Anti-MASP-2 Antibody (B-10) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-MASP-2 Antibody (B-10) is a mouse monoclonal IgG1 (kappa light chain) MASP-2 antibody provided at 200 µg/ml
    • raised against amino acids 284-343 mapping within an internal region of MASP-2 of human origin
    • MASP-2 Antibody (B-10) is recommended for detection of MASP-2 A chain of human origin by WB, IP, IF and ELISA
    • Anti-MASP-2 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MASP-2 (B-10): sc-390200.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MASP-2 Antibody (B-10) for WB applications. These reagents are now offered in bundles with MASP-2 Antibody (B-10) (see ordering information below).
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For Western Blot, is it recommended to use denatured or non-denatured conditions with MASP-2 (B-10): sc-390200 monoclonal antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced positive Western blot data of MASP Produced positive Western blot data of MASP-2 expression in non-transfected and transfected MASP-2 Overexpression CMV whole cell lysates. -SCBT QC
Date published: 2013-09-18
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