Anti-MASP-2 Antibody (B-10) is a mouse monoclonal IgG1 (kappa light chain) MASP-2 antibody provided at 200 µg/ml
raised against amino acids 284-343 mapping within an internal region of MASP-2 of human origin
MASP-2 Antibody (B-10) is recommended for detection of MASP-2 A chain of human origin by WB, IP, IF and ELISA
Anti-MASP-2 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MASP-2 (B-10): sc-390200.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
MASP-2 Antibody (B-10) is a high quality monoclonal MASP-2 antibody (also designated MASP-2 antibody) suitable for the detection of the MASP-2 protein of human origin. MASP-2 Antibody (B-10) is available as both the non-conjugated anti-MASP-2 antibody form, as well as multiple conjugated forms of anti-MASP-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Mannose (or mannan)-binding lectin (MBL), also known as serum mannose-binding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, three types of MBL-associated serine proteases, MASP-1, MASP-2 and MASP-3, and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. MASP-3 is an alternatively spliced product from the MASP-1 gene and may function to inhibit MASP-2 by competing for MBL binding and inhibiting the activation of MBL-associated MASP-2.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For Western Blot, is it recommended to use denatured or non-denatured conditions with MASP-2 (B-10): sc-390200 monoclonal antibody?
Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 5 out of
Produced positive Western blot data of MASPProduced positive Western blot data of MASP-2 expression in non-transfected and transfected MASP-2 Overexpression CMV whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn