Anti-MASP-1/3 Antibody (F-12) recommended for detection of MASP-1/3 heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MASP-1/3 Antibody (F-12): sc-166815

Anti-MASP-1/3 Antibody (F-12) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-MASP-1/3 Antibody (F-12) is a mouse monoclonal IgG2a κ MASP-1/3 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 171-430 mapping within an internal region of MASP-1 of human origin
    • Anti-MASP-1/3 Antibody (F-12) is recommended for detection of MASP-1/3 heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-MASP-1/3 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MASP-1/3 (F-12): sc-166815.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MASP-1/3 Antibody (F-12) for WB applications. These reagents are now offered in bundles with MASP-1/3 Antibody (F-12) (see ordering information below).
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What blocking solution should I use with MASP-1/3 (F-12): sc-166815 monoclonal antibody for Western blot?

Asked by: Cweed
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from Does not work with western blotting This antibody does not work with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from Produced positive Western blot data of full Produced positive Western blot data of full-length human recombinant MASP-1. -SCBT QC
Date published: 2014-05-15
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