m-IgGλ BP-HRP: sc-516132
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- mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Horseradish Peroxidase (HRP)
- supplied at 200 µg in 0.5 ml volume
- Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
- Suitable for binding to most, but not all, mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain antibodies
- Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
- For a Cruz Marker™ compatible mouse IgG lambda binding protein, use m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM) (Click here for datasheet)
- For mouse IgGκ immunoglobulins we recommend m-IgGκ BP-HRP (sc-516102)
- Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
- Also see our new m-IgGλ BP-FITC, PE, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.
QUICK LINKS
SEE ALSO...
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
We didn't know our primary antibody subtype ( kappaor lamda), which 2nd we should order?
Thank you for your question. The significant majority of mouse monoclonal IgG primary antibodies (98%) have a kappa light chain. And therefore we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for Western blotting or immunohistochemistry.
https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Only approximately 2% of mouse IgG contains a lambda light chain, but for those subtypes we do offer a lambda-specific mouse IgG binding protein, m-IgGλ BP-HRP: sc-516132.
Date published: 2018-09-13
I was looking for an anti-rat secondary antibody to be used in WB, but I couldn´t find it. I need the secondary antibody for the primary antibodies: sc-58605, sc-58607, and sc-58596. Can you give me an alternative?
Thank you for your question. Unfortunately, we do not currently offer any secondary antibodies specific for detection of rat immunoglobulins. We are working on developing new products, but these are not yet available. We recommend using any conventional anti-rat IgG secondary antibodies for detection of the primary antibodies you have referenced above.
Date published: 2017-01-11
Rated 4 out of
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erik999 from
western blot
We have tested it in WB analysis and it works well. Recommend concentration 1:3000.
Date published: 2021-06-23
Rated 5 out of
5
by
Anonymous from
nice
Not long ago, I bought this product, which was shipped in time and well packaged.It's suitable for my experiment. it's very helpful to carry out the experiment.
Date published: 2021-06-03
Rated 5 out of
5
by
t888 from
great as alternative for secondary antibody
this is the best alternative for serving as secondary antibody
Date published: 2020-12-01
Rated 4 out of
5
by
Liu Liu from
Western blotting
Use 1:5000 dilution and works great for mouse liver lysates
Date published: 2018-01-15
Rated 4 out of
5
by
Mukta S from
Works well
This antibody worked very well in several of my WB experiments.
Date published: 2017-12-19
Rated 5 out of
5
by
chen Y from
good result on WB
The m-IgGλ BP-HRP antibody works well in Werstern Blot detection using Rat articular chondrocytes cell at 1:800. we got a clear protein band.
Date published: 2017-07-14
Rated 4 out of
5
by
lin05 from
Good AB
It is good for WB and always get strong signaling at 1:5000.
Date published: 2017-07-06
Rated 5 out of
5
by
yyyyyy from
good
It is very sensitive ，works very well in my experiments
Date published: 2017-07-06