m-IgG kappa BP-FITC mouse IgG kappa binding protein (m-IgGκ BP) conjugated to fluorescein (FITC), for WB, IF, IHC(P) and FCM

m-IgGκ BP-FITC: sc-516140

m-IgGκ BP-FITC is rated 4.6 out of 5 by 37.
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Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to fluorescein (FITC)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to about 46%, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • While we continue to offer conventional fluorescein (FITC) conjugates (525 nm emission) for immunofluorescent cell staining, we strongly recommend CruzFluor™ 488 Binding Protein (525 nm emission) as an alternative and much superior reagent.
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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I am planning to multicolor-immunostain cultured hippocampal neurons using sc-516140. Would sc-516140 cross-react the IgG kappa light chain of other species (e.g., goat, rabbit, guinea pig, chicken-IgY, etc) and cause non-specific signals?

Asked by: Nijima
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the RRID for this antibody? Need this information in our paper. Thank you!

Asked by: Fang Wang
SCBT Is not responsible for the curation or assignment of RRID numbers for our products. This is an initiative from a consortium of researchers and The Antibody Registry. More information can be found on the website below. Http://antibodyregistry.org/
Answered by: Tech Service 1
Date published: 2021-02-04

This protein bind to IgGk. Does this mean the tertiary structure of IgG.

Asked by: yapyap
Thank you for your question. This binding protein is specific against the kappa light chain of IgG, IgA and IgM antibodies, which is a small polypeptide subunit of those immunoglobulins.
Answered by: Tech Support Europe
Date published: 2020-11-27

Can the these antibodies be labeled with m-IgIK BP-FITC: sc-52219, sc-52221, sc-52223, sc-57716, sc-71004? I didn't see kappa light chain in their descriptions.

Asked by: Christian_RTP
Thank you for your question. Yes, this secondary antibody can be used for detection with each of the primary antibodies listed. We confirmed these primary antibodies are all kappa light chain type. This information will be updated in their descriptions on our website.
Answered by: Technical Support
Date published: 2020-04-09

May i know this secondary antibody sc516140 is raised in goat or rabbit?thank you

Asked by: Fazlin
Thank you for your question. This item, sc-516140 is a mouse IgG kappa binding protein conjugated to FITC. It is not raised in goat or rabbit.
Answered by: Tech Service
Date published: 2019-10-23

Can m-IgGκ BP-FITC: sc-516140 be used for western blotting? and if yes how can bands be visualized

Asked by: Yahia
Thank you for your question. Yes, m-IgGκ BP-FITC: sc-516140, can be used for Western blotting as long as you have capabilities to visualize the results. FITC is a fluorochrome and you need to have the ability to see the resulting bands. We do not actually recommend sc-516140 for WB, so I would suggest using a CruzFluor conjugated secondary instead, which can be visualized using for example LiCor. You can find WB recommended secondaries here: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-10-03

Can I use sc-516140 (FITC) and sc-3598 (DAPI) with these first antibodies? sc-32233 sc-130335 sc-271548 sc-365977 sc-390818 Can I use it with some antibodies from thermofisher?

Asked by: Dr Tomasi
Thank you for your question.Yes, our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to fluorescein (FITC) can be used as a secondary detection antibody with each of the primary mouse monoclonal IgG (kappa light chain) antibodies from SCBT that you have inquired about. DAPI can be used as a fluorescent nuclear stain and is compatible with any antibodies that are recommended for IF (immunofluorescence). Yes, our m-IgGκ BP-FITC can be used as a secondary detection antibody with antibodies from other suppliers. Specifically, any primary antibodies which are mouse monoclonal IgG or IgM with kappa light chain.
Answered by: Technical Support
Date published: 2019-04-13

I have a primary antibody from Santa Cruz Technology, unconjugated mouse monoclonal SHIP-1 P1C1 sc-8425.Would this antibody (m-IgGκ BP-FITC: sc-516140) work as a secondary antibody for this primary for immunofluorescence?

Asked by: Bradley187
Thank you for your question. Yes, this is a suitable secondary for detection of that primary antibody. SHIP-1 Antibody (P1C1) is a mouse monoclonal IgG1 (kappa light chain), so our mouse IgG kappa binding protein will recognize it specifically.
Answered by: Technical Support
Date published: 2019-04-08
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Rated 4 out of 5 by from 免疫荧光 早期申请过santa该产品试用装,用于免疫荧光,着色还可以,1:100-200稀释,性价比还是比较高的。
Date published: 2021-06-23
Rated 5 out of 5 by from Epigenome Editing Not long ago, I bought this product, which was shipped in time and well packaged.It's suitable for my experiment. it's very helpful to carry out the experiment.
Date published: 2021-06-03
Rated 4 out of 5 by from Secondary Antibody for flow cytometry This antibody worked really well for flow cytometry
Date published: 2018-05-04
Rated 4 out of 5 by from Works fine I got this antibody as a free sample. It worked well for immunofluorescence applications but had some background.
Date published: 2017-12-01
Rated 5 out of 5 by from Works fine for cryosections! I used this antibody 1:80 for cryosections of mouse adrenals, with chromogranin A (sc-393941) 1:60 as primary. Works fine!
Date published: 2017-12-01
Rated 5 out of 5 by from 强烈推荐santa抗体 这是我申请的试用装,染色效果很好,强烈推荐,货号SC-516140 SC-7273 ,进行流式检测,需要破核膜染色
Date published: 2017-11-28
Rated 5 out of 5 by from superb IHF shows positive results in our experiments , good very good.
Date published: 2017-10-31
Rated 5 out of 5 by from Worked well IFC results were clear and represent itself as good material for the accomplishment of IFC staining for mouse aortic tissue and cultured VSMC in vitro.
Date published: 2017-10-27
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