LXR alpha / beta Antibody (H-7)recommended for detection of LXRα and LXRβ of mouse, rat, human and avian origin by WB, IP, IF and ELISA

LXRα/β Antibody (H-7): sc-377260

LXRα/β Antibody (H-7) is rated 4.5 out of 5 by 8.
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Datasheets

    • LXRα/β Antibody (H-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 433-461 at the C-terminus of LXRβ of human origin
    • LXR alpha / beta Antibody (H-7) is recommended for detection of LXRα and LXRβ of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • LXR alpha / beta Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-377260 X, 200 µg/0.1 ml)
    • blocking peptide, sc-377260 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of LXRα/β (H-7): sc-377260.
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What application is the blocking peptide sc-377260 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent in WB Western blotting dilution 1:1000 C2C12 cell lysate specific and very clear band
Date published: 2018-10-05
Rated 2 out of 5 by from CHIP failed with sample I applied a sample to do the CHIP experiment, the sample was the mouse liver, the ultrasound fragmented between 300-800bp, each sample used 3ug antibody, the results shown in the figure
Date published: 2017-09-12
Rated 4 out of 5 by from working good! I got this two weeks ago and used 1:1000. working good on my WB.
Date published: 2017-07-21
Rated 5 out of 5 by from great features WB 1:500 on mouse liver samples,band is very clear
Date published: 2017-05-08
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of LXRα/β expression in HeLa and THP-1 whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from Worked well for co-IP in mouse macrophages We get pretty results in IP assay, our samples are mouse macrophages, The working dilution is 1: 100.
Date published: 2017-01-15
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Good specificity in WB in human LXR transfected Good specificity in WB in human LXR transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-14
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