Lucigenin CAS: 2315-97-1
MF: C28H22N4O6
MW: 510.50
A chemiluminescent superoxide probe.

Lucigenin (CAS 2315-97-1)

Lucigenin | CAS 2315-97-1 is rated 5.0 out of 5 by 1.
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Alternate Names: 9,9′-Bis(N-methylacridinium nitrate); N,N′-Dimethyl-9,9′-biacridinium dinitrate
Application: Lucigenin is a chemiluminescent superoxide probe
CAS Number: 2315-97-1
Purity: ≥97%
Molecular Weight: 510.50
Molecular Formula: C28H22N4O6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Lucigenin is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells. Upon single-electron reduction of Lucigenin by a radical species the subsequent form of Lucigenin presents an amplified chemiluminescence which is measurable and can be correlated to the respective amount of reactive radical species present in the system. This approach can be used to probe the natural generation of these species as well as the natural functioning of superoxide dismutase and other enzymes responsible for scavenging radicals. In addition to indicating the presence of radicals in cells, Lucigenin shows interaction with redox-active oxidoreductase enzymes. Lucigenin is shown to mediate the reduction of cytochrome C by xanthine oxidase and to increase the rate of NADPH oxidation. These observations indicate that use of Lucigenin for superoxide analysis is precluded in the presence of high levels of NAD(P)H-oxidoreductases.


References

1. Li, Y., et al. 1998. J. Biol. Chem. 273: 2015-2023. PMID: 9442038
2. Rost, M., et al. 1998. J. Biolumin. Chemilumin. 13: 355-363. PMID: 9926363
3. Afanas'ev, I.B., et al. 1999. Arch. Biochem. Biophys. 366: 267-274. PMID: 10356292
4. Skatchkov, M.P., et al. 1999. Biochem. Biophys. Res. Commun. 254: 319-324. PMID: 9918836
5. Schepetkin, I.A. 1999. Biochemistry Mosc. 64: 25-32. PMID: 9986909

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (~12 mg/ml), 100% ethanol (~0.5 mg/ml), acetic acid (~10 mg/ml), and water (8 mg/ml).
Storage :
Store at room temperature
Melting Point :
245-250° C
Fluoresence :
λex 319 nm, λem 384 nm (pH 9.0); λex 360 nm, λem 449 nm (Reaction Product); λex 368 nm, λem 505 nm in water; λex 455 nm, λem 505 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
MDL Number :
MFCD00011925
EC Number :
219-023-4
Beilstein Registry :
3901768
SMILES :
O[N+]([O-])=O.O[N+]([O-])=O.C[N+]1=C2C=CC=CC2=C(C2=CC=CC=C12)C1=C2C=CC=CC2=[N+](C)C2=CC=CC=C12

Download SDS (MSDS)

Certificate of Analysis

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Lucigenin (CAS 2315-97-1)  Product Citations

See how others have used Lucigenin (CAS 2315-97-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 14 total

PMID: # 28697598  Zheng, K. et al. 2017. J. Agric. Food Chem. 65: 6625-6637.

PMID: # 25798836  Choi, JA. et al. 2016. Oncogene. 35: 83-93.

PMID: # 26906075  Asensio-López, MC. et al. 2016. Arch. Biochem. Biophys. 594: 26-36.

PMID: # 27310928  Zheng, K. et al. 2016. Autophagy. 0.

PMID: # 26859849  Silva, GB. et al. 2016. Nephron. 132: 153-60.

PMID: # 25048575  Botz, B. et al. 2014. 66: 2739-50.

PMID: # 25112872  Tamma, G. et al. 2014. J. Biol. Chem. 289: 27807-13.

PMID: # 23824088  Li, FC. et al. 2013. Toxicol. Sci. 135: 202-17.

PMID: # 23533503  Jin, HJ. et al. 2013. Evid Based Complement Alternat Med. 2013: 610694.

PMID: # 23994555  Wang, C. et al. 2013. Toxicol. Appl. Pharmacol. 273: 59-67.

Citations 1 to 10 of 14 total
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Rated 5 out of 5 by from Schepetkin (PubMed ID 9986909) found an elevated Schepetkin (PubMed ID 9986909) found an elevated rate of NADPH reduction by microsomal NADPH reductase in the presence of lucigenin. -SCBT Publication Review
Date published: 2015-06-07
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