Anti-Luciferase Antibody (C-12) recommended for detection of Luciferase of Photinus pyralis origin by WB, IP, IF and ELISA

Anti-Luciferase Antibody (C-12): sc-74548

Anti-Luciferase Antibody (C-12) is rated 4.5 out of 5 by 13.
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Datasheets

    • Anti-Luciferase Antibody (C-12) is a mouse monoclonal IgG2a κ Luciferase antibody, cited in 17 publications, provided at 200 µg/ml
    • raised against amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin
    • Luciferase Antibody (C-12) is recommended for detection of Luciferase of Photinus pyralis origin by WB, IP, IF and ELISA
    • Anti-Luciferase Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Luciferase (C-12): sc-74548.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Luciferase Antibody (C-12) for WB applications. These reagents are now offered in bundles with Luciferase Antibody (C-12) (see ordering information below).
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Does this antibody react with nanoLuc luciferase from Promega?

Asked by: JC2020
Thank you for your question. This mouse monoclonal Luciferase antibody, sc-74548 was raised against amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin. Unfortunately we do not have any information regarding the nanoLuc Luciferase from Promega, but it does detect standard Luciferace of Photinus pyralis origin.
Answered by: Tech Service
Date published: 2020-01-27

Concerning the IP antibody sc-74548 AC, what kind of bound is it between the agarose beads and the antibody ? A covalent one ?

Asked by: dmr56re
Thank you for your question. Yes, the antibody is covalently bound to the agarose beads via the primary amines.
Answered by: Tech Service
Date published: 2020-01-10

Hi I have a split luciferase expression system where one vector has nLUC (N terminal LUC) and another vector has cLUC (C terminal LUC). I want to know whether, this antibody can detect both N and C terminal LUC? Thanks

Asked by: skarre86
Thank you for your question. This antibody has a C-terminal epitope so it will detect the protein that contains the C-terminal region. Please contact technical service if you need further assistance.
Answered by: Tech Service
Date published: 2019-02-08

Hi, I want to perform western blotting for a modified firefly luciferase p2luc, just wondering if this antibody will work with this modified P2luc

Asked by: vindu
Thank you for your question. Please contact our Technical Service Department and we can assist you by performing sequence analysis of the antigen used to develop the antibody, along with your specific modified p2luc protein sequence.
Answered by: Technical Support
Date published: 2019-01-26

Is there a blocking peptide available for this antibody?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, it is not possible to offer a blocking peptide for this product.
Answered by: Technical Support 7
Date published: 2017-01-11

What is the recommended secondary reagent for this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-11
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Rated 5 out of 5 by from Works perfectly for WB works very well in WB, incubation in PBS-T Milk 5% 1h at 4°C.
Date published: 2021-07-15
Rated 4 out of 5 by from funziona bene Questo anticorpo produce una banda singola e netta.
Date published: 2019-05-15
Rated 5 out of 5 by from great firefly luciferase. Loading 20ug, antibody 1:500 dilution, get band at 62kD. good.
Date published: 2018-01-26
Rated 5 out of 5 by from Excellent results the sample lucferase (C-12) sc-74548 works very well, we did already buy for our lab.
Date published: 2017-11-13
Rated 5 out of 5 by from Excellent antibody I purchased this antibody two weeks ago. It works very well for western blot analysis.
Date published: 2017-10-27
Rated 5 out of 5 by from Positive result Nice sample antibody.Excelent results by WB on huamn skeletal muscle 1:700 used.
Date published: 2017-04-29
Rated 5 out of 5 by from Great Luciferase detection Great antibody for luciferase detection by WB, IP, IF and ELISA. Published results in multiple applications; highly recommended.
Date published: 2017-01-23
Rated 5 out of 5 by from Great product Very nice western blot results; strong signal with low background
Date published: 2017-01-15
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