LPS A mitogen for bone marrow derived B lymphocytes.

LPS

LPS is rated 5.0 out of 5 by 6.
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Alternate Names: LPS is also known as Lipopolysaccharide.
Application: LPS is a highly immunogenic major component of the cell wall of gram negative bacteria that strongly activates immune cells bearing the CD14/TLR4/MD2 receptor complex.
Purity: purified by phenol extraction
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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LPS (Lipopolysaccharide) is an endotoxin which exists as a critical glycolipid component of the outer cell wall of gram-negative bacteria. LPS is a known, potent initiator of the proinflammatory response and induces proinflammatory cytokines in several cell types. Many of the cell signals induced by gram-negative bacteria are attributed to LPS and has been observed to also activate TNF receptor signaling pathways. The major receptor that transduces the LPS signal has been recognized as a member of the Toll-like receptor family. At least 10 members of the family have been identified to date and TLR4 appears to be the main LPS receptor although coreceptors are most likely involved. Studies note that in HMEC (human microvascular endothelial cells) cell lines, LPS activates apoptosis pathways via both NF-κB and JNK through TRAF6. Also reported that NF-κB inhibition does not potentiate LPS-induced death in HMEC cells yet will sensitive these cell to TNF-induced death. Additional studies using HMEC cell lines report that following LPS stimulation, TRAF6 also transmits an important endothelial cell survival signal in a situation of complete NF-κB blockade which may indicate that TRAF6 signals an NF-κB–independent antiapoptotic pathway in endothelial cells exposed to LPS. LPS is also known as Lipopolysaccharides, Lipopolysaccharides from E. coli O111:B4, and Bacterial lipopolysaccharides.


References

1. Zhang, F.X., et al. 1999. J. Biol. Chem. 274: 7611-7614. PMID: 10075645

2. Hull, C., et al. 2002. J. Immunol. 169: 2611-2618. PMID: 12193732

3. Raschi, E., et al. 2003. Blood. 101: 3495-3500. PMID: 12531807

4. Pollet, I., et al. 2003. Blood. 102: 1740-1742. PMID: 12714497

5. Wong, F., et al. 2004. Blood. 103: 4520-4526. PMID: 14996708

6. Wang, J., et al. 2008. J. Immunol. 180: 8011-8019. PMID: 18523264

Usage :
For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solution or cell culture medium.
Storage/Stability:
Solutions at 1 mg/ml in buffer or culture medium are stable for ~1 month at 2–8° C. Frozen aliquots can be stored up to 2 years. Repeated freeze/thaw cycles are not recommended. Solutions should be stored in silanized containers, since LPS can bind to plastics and certain types of glass (especially at concentrations of <0.1 mg/ml). If the LPS concentration is >1 mg/ml, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve the adsorbed product.
Appearance :
Lyophilized powder
Physical State :
Solid
Derived From :
E. coli O111:B4
Solubility :
Soluble in water (5 mg/ml), and cell culture medium (1 mg/ml).
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
MDL Number :
MFCD00164401
SMILES :
CCCCCCCCCCCCCC(=O)O[[email protected]](CCCCCCCCCCC)CC(=O)O[[email protected]@H]1[[email protected]]([[email protected]@H](O[[email protected]@H]([[email protected]]1OP(=O)(O)O)CO[[email protected]@]2(C[[email protected]]([[email protected]@H]([[email protected]](O2)[[email protected]](CO)O)OC3[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O3)[[email protected]](CO)O)OP(=O)(O)OP(=O)(O)OCCN)OC4[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O4)[[email protected]](COC5[[email protected]]([[email protected]]([[email protected]@H]([[email protected]](O5)[[email protected]](CO)O)OP(=O)(O)O)O)O)O)O)OC6[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O6)CO[[email protected]@H]7[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O7)CO)O)O)O)O)O[[email protected]@H]8[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O8)CO)O)O)OC9[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O9)CO)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO[[email protected]@H]1[[email protected]@H]([[email protected]]([[email protected]]([[email protected]](O1)CO)O)O)O)O)O)O)NC(=O)C)NC(=O)C)O[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O)O)NC(=O)C)O)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)O[[email protected]@]1(C[[email protected]]([[email protected]@H]([[email protected]](O1)[[email protected]](CO)O)O)OP(=O)(O)OCCN)C(=O)O)C(=O)O)C(=O)O)OC[[email protected]@H]1[[email protected]]([[email protected]@H]([[email protected]]([[email protected]](O1)OP(=O)(O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)O)OC(=O)C[[email protected]@H](CCCCCCCCCCC)O)O)NC(=O)C[[email protected]@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC

Download SDS (MSDS)

Certificate of Analysis

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LPS  Product Citations

See how others have used LPS. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 75 total

PMID: # 34192597  Pazoki, H.|Mohammad Rahimi, H.|Mirjalali, H.|Niyyati, M.|Mosaffa, N.|Seyed Tabaei, SJ.|Shahrokh, S.|Asadzadeh Aghdaei, H.|Zali, MR.| et al. 2021. Microb Pathog. 158: 105072.

PMID: # 34494097  Vestre, K.|Persiconi, I.|Distefano, MB.|Mensali, N.|Guadagno, NA.|Bretou, M.|Wälchli, S.|Arnold-Schrauf, C.|Bakke, O.|Dalod, M.|Lennon-Dumenil, AM.|Progida, C.| et al. 2021. J Cell Sci.

PMID: # 32135404  Xu, X.|Hu, P.|Ma, Y.|Tong, L.|Wang, D.|Wu, Y.|Chen, Z.|Huang, C.| et al. 2020. J Nutr Biochem. 78: 108323.

PMID: # 30938885  Yang, J.|Li, R.|Zhao, D.|Zheng, S.| et al. 2019. J. Cell. Biochem. 120: 12887-12903.

PMID: # 30914954  Rossaneis, AC.|Longhi-Balbinot, DT.|Bertozzi, MM.|Fattori, V.|Segato-Vendrameto, CZ.|Badaro-Garcia, S.|Zaninelli, TH.|Staurengo-Ferrari, L.|Borghi, SM.|Carvalho, TT.|Bussmann, AJC.|Gouveia, FS.|Lopes, LGF.|Casagrande, R.|Verri, WA.| et al. 2019. Front Pharmacol. 10: 229.

PMID: # 29581852  Wang, Q.|Yang, X.|Xu, Y.|Shen, Z.|Cheng, H.|Cheng, F.|Liu, X.|Wang, R.| et al. 2018. Oncotarget. 9: 14397-14412.

PMID: # 29058362  Huang, C. et al. 2018. Glia. 66: 256-278.

PMID: # 30247686  Miklavcic, JJ.|Badger, TM.|Bowlin, AK.|Matazel, KS.|Cleves, MA.|LeRoith, T.|Saraf, MK.|Chintapalli, SV.|Piccolo, BD.|Shankar, K.|Yeruva, L.| et al. 2018. J. Nutr. 148: 1860-1870.

PMID: # 29844724  Al-Saeedan, AS.|Gautam, V.|Ansari, MN.|Singh, M.|Yadav, RK.|Rawat, JK.|Devi, U.|Gautam, S.|Roy, S.|Kaithwas, G.| et al. 2018. Saudi Pharm J. 26: 520-527.

PMID: # 28892097  Muku, GE.|Lahoti, TS.|Murray, IA.|Podolsky, MA.|Smith, KJ.|Hubbard, TD.|Kuzu, G.|Gowda, K.|Amin, SG.|Perdew, GH.| et al. 2017. Lab Invest. 97: 1471-1487.

Citations 1 to 10 of 75 total

What is the physical appearance?

Asked by: SCM4
Previous lots have been faint yellow in color.
Answered by: Tech Service 11
Date published: 2018-08-06
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Rated 5 out of 5 by from Very well We experimentally determined that many of the cellular signals caused by Gram negative bacteria are due to LPS and have also been observed to activate the TNF receptor signaling pathway.
Date published: 2017-02-09
Rated 5 out of 5 by from lipopolysaccharide We have determined that lipopolysaccharide is an endotoxin present as a key glycolipid component of the outer cell wall of gram-negative bacteria, although the compound is known, it can be measured as an effective proinflammatory agent, and in several cells Type induces proinflammatory cytokines.
Date published: 2017-02-03
Rated 5 out of 5 by from Arrived quickly Arrived quickly - performed as expected.
Date published: 2015-10-08
Rated 5 out of 5 by from Used and worked well Used and worked well.
Date published: 2015-09-09
Rated 5 out of 5 by from Works great in our experiments Works great in our experiments, no complaints.
Date published: 2015-03-25
Rated 5 out of 5 by from An increase in cleaved caspase An increase in cleaved caspase-1 (p10) was observed by WB in THP-1 cells post treatment with LPS. -SCBT QC
Date published: 2015-02-01
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