Lnk Antibody (A-12)recommended for detection of Lnk of mouse, rat and human origin by WB, IP, IF and ELISA

Lnk Antibody (A-12): sc-393709

Lnk Antibody (A-12) is rated 4.3 out of 5 by 3.
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Datasheets

    • Lnk Antibody (A-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 447-575 mapping at the C-terminus of Lnk of human origin
    • Lnk Antibody (A-12) is recommended for detection of Lnk of mouse, rat and human origin by WB, IP, IF and ELISA
    • Lnk Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Lnk (A-12): sc-393709.
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Hi, We tried to do IP with Lnk (A-12) Ab. We used suggested Ab:protein ratio and a commercial kit for MS (e.g. we do not know buffers composition). We did not get any Lnk bands in our IP. Is this Ab suitable for the IP? Do you have a protocol? Thank you

Asked by: ysia2000
Thank you for your question. Lnk Antibody (A-12): sc-393709 is recommended for detection of Lnk of mouse, rat and human origin by WB, IP, IF and ELISA, so it is suitable for immunoprecipitation. You can find our recommended protocol by following this link: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots Feel free to contact our technical service if you need further assistance with your experiment.
Answered by: Tech Support Europe
Date published: 2019-05-28

What is the recommended fixation method for immunofluorescence staining with Lnk (A-12): sc-393709 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good but had some background Good but had some background.
Date published: 2015-03-19
Rated 5 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane and cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Satisfactory Western Blot data of Lnk expression Satisfactory Western Blot data of Lnk expression in Jurkat, Ramos, MOLT-4 and WEHI-231 whole cell lysates. -SCBT QC
Date published: 2014-01-19
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