Laminin γ-1 Antibody (D-3): sc-17751

Laminin γ-1 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Laminin γ-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Laminin γ-1 antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminins, including Laminin γ-1, are crucial structural components of basement membranes, which are specialized extracellular matrices that provide support and anchorage for cells. These membranes are not only vital for maintaining tissue architecture but also play a significant role in regulating cellular behaviors such as proliferation, migration, and differentiation. The unique structure of laminins, which consists of heterotrimers formed by alpha, beta, and gamma chains, allows them to self-assemble and interact with other matrix components, facilitating the formation of stable basement membranes. The human laminin gamma-1 gene is located on chromosome 1q31 and is expressed in various tissues, highlighting its importance in the development and maintenance of tissues that require a robust extracellular matrix. Laminin γ-1 (D-3) monoclonal antibody is an essential tool for researchers studying the dynamics of basement membranes and their impact on cellular functions in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Laminin γ-1 Antibody (D-3) References:

1. bcn-1 Element-dependent activation of the laminin gamma 1 chain gene by the cooperative action of transcription factor E3 (TFE3) and Smad proteins.  |  Kawata, Y., et al. 2002. J Biol Chem. 277: 11375-84. PMID: 11801598
2. The critical role of basement membrane-independent laminin gamma 1 chain during axon regeneration in the CNS.  |  Grimpe, B., et al. 2002. J Neurosci. 22: 3144-60. PMID: 11943817
3. Laminin gamma 1 chain peptide, C-16 (KAFDITYVRLKF), promotes migration, MMP-9 secretion, and pulmonary metastasis of B16-F10 mouse melanoma cells.  |  Kuratomi, Y., et al. 2002. Br J Cancer. 86: 1169-73. PMID: 11953867
4. Plasmin-mediated degradation of laminin gamma-1 is critical for ethanol-induced neurodegeneration.  |  Skrzypiec, AE., et al. 2009. Biol Psychiatry. 66: 785-94. PMID: 19577227
5. Autoantibodies Against Multiple Epitopes in Bp180 and Laminin Gamma-1 in Subepidermal Blistering Skin Disease Associated with Psoriatic Erythroderma.  |  Goto-Hamano, H., et al. 2015. Indian J Dermatol. 60: 521. PMID: 26538726
6. A Serological Biomarker of Laminin Gamma 1 Chain Degradation Reflects Altered Basement Membrane Remodeling in Crohn's Disease and DSS Colitis.  |  Lindholm, M., et al. 2022. Dig Dis Sci. 67: 3662-3671. PMID: 34561759
7. Oligodendrocyte-derived laminin-γ1 regulates the blood-brain barrier and CNS myelination in mice.  |  Kang, M. and Yao, Y. 2024. Cell Rep. 43: 114123. PMID: 38635399
8. Two non-contiguous regions contribute to nidogen binding to a single EGF-like motif of the laminin gamma 1 chain.  |  Pöschl, E., et al. 1994. EMBO J. 13: 3741-7. PMID: 8070402
9. Expression of laminin gamma 1 cultured hepatocytes involves repeated CTC and GC elements in the LAMC1 promoter.  |  Levavasseur, F., et al. 1996. Biochem J. 313 (Pt 3): 745-52. PMID: 8611150
10. Cloning of rat laminin gamma 1-chain gene promoter reveals motifs for recognition of multiple transcription factors.  |  O'Neill, BC., et al. 1997. Am J Physiol. 273: F411-20. PMID: 9321914

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Laminin γ-1 Antibody (D-3) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Laminin γ-1 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Laminin γ-1 Antibody (D-3) Specifications

Alternate Product Name: Laminin γ-1

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1420-1609

Epitope Target: Laminin γ-1

Epitope Species: human

Product Reactivity/Detection: Laminin γ-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Laminin γ-1 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Laminin γ-1 antibody is Laminin γ-1 Antibody (B-4): sc-13144.