Anti-KOR-1 Antibody (D-8) recommended for detection of KOR-1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-KOR-1 Antibody (D-8): sc-374479

Anti-KOR-1 Antibody (D-8) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-KOR-1 Antibody (D-8) is a mouse monoclonal IgG2b κ KOR-1 antibody, cited in 6 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 351-380 within a C-terminal cytoplasmic domain of KOR-1 of human origin
    • Anti-KOR-1 Antibody (D-8) is recommended for detection of KOR-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-KOR-1 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of KOR-1 (D-8): sc-374479.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KOR-1 Antibody (D-8) for WB applications. These reagents are now offered in bundles with KOR-1 Antibody (D-8) (see ordering information below).
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Says this antibody is recommended for the detection of mouse KOR and can be used with immunofluorescence. Just wondering if there are any additional steps recommended for using anti-mouse antibody on mouse tissue?

Asked by: Kelly1234
Thank you very much for the question! For IF staining of IgG-rich mouse Samples we suggest using our conjugated Mouse IgG kappa binding proteins e.g. Conjugated to CFL 488 sc-516190 or -FITC instead of conventional anti-mouse secondary antibodies. Our labs have excellent experience with this reagent and we have a lot of supporting customer feedback as well. Of course you always also could place a special order for a directly -CFL488 conjugated KOR primary antibody with our Special Orders department. A directly conjugated primary antibody will of course be a suitable approach as well.
Answered by: Technical Support Europe
Date published: 2017-09-18

How should the blocking peptide sc-374479 P be used? What is the protocol?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control. The full protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by from Strong banding for Western blot KOR-1 (D-8) gives a great band in positive control PC-12 Cell Lysate: sc-2250.
Date published: 2017-01-11
Rated 5 out of 5 by from Great Wonderful Antibody , ured it for immunohistochemistry slide of mouse retina, got wonderful pictures.
Date published: 2016-12-11
Rated 5 out of 5 by from Produced nice Western blot data of KOR Produced nice Western blot data of KOR-1 expression in PC-12 whole cell lysate. -SCBT QC
Date published: 2014-07-29
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