kleisin β Antibody (B-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 437-606 mapping at the C-terminus of kleisin β of human origin
kleisin beta Antibody (B-1) is recommended for detection of kleisin β of mouse, rat and human origin by WB, IP, IF and ELISA
kleisin beta Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of kleisin β (B-1): sc-393333.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Chromosome formation and subsequent function require the activity of two condensin complexes, namely condensin I and condensin II, both of which are crucial for proper mitotic chromosome assembly and segregation. Kleisin β, also known as NCAPH2 (non-SMC condensin II complex, subunit H2) or CAPH2, is a 605 amino acid protein that localizes to the nucleus and is specifically distributed along the arms of assembled chromosomes. Existing as a component of the condensin II complex, kleisin β functions as a regulatory protein that assists in establishing mitotic chromosome architecture, possibly by providing an increased level of organization and rigidity to formed chromosomes. In response to DNA damage, kleisin β is subject to phosphorylation by ATM or ATR. Multiple isoforms of kleisin β exist due to alternative splicing events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
According to NCBI and Ensembl.org the largest CAP-H2/Kleisin-beta isoforms are 68 kDa. Can you explain why your blots with this antibody only show bands larger than 90kDa?
Asked by: Christian Nielsen
Thank you for your question. Please refer to the following publication for more detailed support of the ~90 kDa size of kleisin β, also designated hCAP-H2, PMID: 14532007.
Answered by: Technical Support
Date published: 2019-05-25
What is the recommended fixation method for immunofluorescence staining with kleisin β (B-1): sc-393333 monoclonal antibody?
Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.