kleisin beta Antibody (B-1)recommended for detection of kleisin β of mouse, rat and human origin by WB, IP, IF and ELISA

kleisin β Antibody (B-1): sc-393333

kleisin β Antibody (B-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • kleisin β Antibody (B-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 437-606 mapping at the C-terminus of kleisin β of human origin
    • kleisin beta Antibody (B-1) is recommended for detection of kleisin β of mouse, rat and human origin by WB, IP, IF and ELISA
    • kleisin beta Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of kleisin β (B-1): sc-393333.
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According to NCBI and Ensembl.org the largest CAP-H2/Kleisin-beta isoforms are 68 kDa. Can you explain why your blots with this antibody only show bands larger than 90kDa?

Asked by: Christian Nielsen
Thank you for your question. Please refer to the following publication for more detailed support of the ~90 kDa size of kleisin β, also designated hCAP-H2, PMID: 14532007.
Answered by: Technical Support
Date published: 2019-05-25

What is the recommended fixation method for immunofluorescence staining with kleisin β (B-1): sc-393333 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Produced positive Western Blot data of kleisin Produced positive Western Blot data of kleisin expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-07-25
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