Kininogen HC Antibody (2B5)recommended for detection of common heavy chain of HMWK of mouse, rat and human origin by WB, IP, IF and IHC(P)

Kininogen HC Antibody (2B5): sc-23914

Kininogen HC Antibody (2B5) is rated 3.8 out of 5 by 4.
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Datasheets

    • Kininogen HC Antibody (2B5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified high molecular weight Kininogen (HMWK) of human origin
    • Kininogen HC Antibody (2B5) is recommended for detection of common heavy chain of HMWK of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Kininogen HC Antibody (2B5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Kininogen HC (2B5): sc-23914.
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I am using Kininogen HC (2B5): sc-23914 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Publishable data in WB using human blood Publishable data in WB using human blood -SCBT Publication Review
Date published: 2015-06-27
Rated 4 out of 5 by from Good band seen in WB using purified human Good band seen in WB using purified human Kininogen. -SCBT QC
Date published: 2015-05-14
Rated 4 out of 5 by from Acceptable immunoperoxidase membrane staining Acceptable immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human platelets. -SCBT QC
Date published: 2015-01-15
Rated 4 out of 5 by from Produced acceptable immunofluorescence cell Produced acceptable immunofluorescence cell surface staining in methanol-fixed ECV304 cells. -SCBT QC
Date published: 2013-02-16
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