KIF20A Antibody (D-3)recommended for detection of KIF20A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

KIF20A Antibody (D-3): sc-374508

KIF20A Antibody (D-3) is rated 4.9 out of 5 by 7.
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Datasheets

    • KIF20A Antibody (D-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 592-890 mapping at the C-terminus of KIF20A of human origin
    • KIF20A Antibody (D-3) is recommended for detection of KIF20A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • KIF20A Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of KIF20A (D-3): sc-374508.
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I am using KIF20A (D-3): sc-374508 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for IF This KIF20A Antibody (D-3) shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Western Blot Buen resultado Western Blot dilución 1:500. 30 ug de las muestras (endometrio y Hela cells)
Date published: 2017-12-05
Rated 5 out of 5 by from wonderful I used this antibody for WB at 1:100 dilution.The bands were clear.
Date published: 2017-11-22
Rated 5 out of 5 by from KIF20A (D-3)的WB结果示条带清晰 上个月从scbt公司官网上订购KIF20A (D-3)抗体,后在肝癌组织中跑WB检验,目标条带清晰,无明显杂带。该抗体工作良好。
Date published: 2017-10-19
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of KIF20A expression in Hep G2 and HeLa whole cell lysates.
Date published: 2017-01-20
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Great Western Blot data of KIF20A expression Great Western Blot data of KIF20A expression in Hep G2 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-10
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