Anti-Keap1 Antibody (F-10) recommended for detection of Keap1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Keap1 Antibody (F-10): sc-514914

Anti-Keap1 Antibody (F-10) is rated 3.8 out of 5 by 4.
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Datasheets

    • Keap1 Antibody (F-10) is a mouse monoclonal IgG2b κ, cited in 14 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 39-65 near the N-terminus of Keap1 of human origin
    • Anti-Keap1 Antibody (F-10) is recommended for detection of Keap1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Keap1 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Keap1 (F-10): sc-514914.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for Keap1 Antibody (F-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Keap1 Antibody (F-10) (see ordering information below).
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How much is the antibody of Keap1 (F-10): sc-514914. Would the free 10ug be available to help us work out some issues?

Asked by: Allan
Thank you for your question. Current pricing is available on our website. Please contact our Customer Care Department for a formal quotation. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-26

How much is shipping for https://www.scbt.com/scbt/product/keap1-antibody-f-10 ? Does this need a secondary antibody? If so can you suggest which one?

Asked by: Allan
This primary antibody ships Express Ambient via FedEx Express standard Overnight. Please refer to the link below for current shipping information. https://www.scbt.com/scbt/customer-care/shipping-information If you purchase the unconjugated mouse monoclonal antibody, sc-514914, then we recommend using our mouse IgG binding protein as a secondary antibody for detection (e.g., sc-516102). We can recommend a specific conjugated secondary antibody based on your intended research application. Please contact Technical Service for support.
Answered by: Technical Support
Date published: 2019-01-26

What application is the blocking peptide sc-514914 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 3 out of 5 by from Good detection for Keap1 antibody The antibody request 30-50 µg of cell lysates and is suitable for many cell lines. Good quality
Date published: 2018-08-28
Rated 3 out of 5 by from Weak detection via Western blot Weak detection via Western blot in human breast cancer cell lines.
Date published: 2018-04-24
Rated 4 out of 5 by from 挺好的 一个月前申请的小样,western blot鉴定效果还不错,条带清晰,挺好的!!!!!!!!!!!!
Date published: 2018-03-04
Rated 5 out of 5 by from Excellent It is an excellent antibody, it works perfectly in WB in protein of Hela cells. Strong single band can be observed.
Date published: 2017-01-18
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