karyopherin beta 2 Antibody (D45)recommended for detection of karyopherin β2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

karyopherin β2 Antibody (D45): sc-32314

karyopherin β2 Antibody (D45) is rated 4.7 out of 5 by 3.
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Datasheets

    • karyopherin β2 Antibody (D45) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against His-tagged karyopherin β2 of human origin
    • recommended for detection of karyopherin β2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can karyopherin β2 (D45): sc-32314 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, karyopherin β2 (D45): sc-32314 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from WB with non WB with non-transfected 293T, human karyopherin 2 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic, membrane and nuclear staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-30
Rated 4 out of 5 by from Positive results with some background in Positive results with some background in western blot analysis of karyopherin 2 expression in HeLa, Hep G2, Jurkat and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-20
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