karyopherin beta 2/2B Antibody (A-11)recommended for detection of karyopherin β2 and karyopherin β2B of mouse, rat, human and avian origin by WB, IP, IF and ELISA

karyopherin β2/2B Antibody (A-11): sc-365179

karyopherin β2/2B Antibody (A-11) is rated 4.8 out of 5 by 4.
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Datasheets

    • karyopherin β2/2B Antibody (A-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of karyopherin β2 of human origin
    • karyopherin beta 2/2B Antibody (A-11) is recommended for detection of karyopherin β2 and karyopherin β2B of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine and avian
    • karyopherin beta 2/2B Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-365179 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin β2/2B (A-11): sc-365179.
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What application is the blocking peptide sc-365179 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for WB it works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Strong bands in western blot analysis of Strong bands in western blot analysis of karyopherin 2/2B expression in Jurkat and A-431 whole cell lysates. -SCBT QC
Date published: 2015-05-22
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-23
Rated 5 out of 5 by from WB: clean WB: clean, clear band present in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-11
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