karyopherin beta 1 Antibody (H-7)recommended for detection of karyopherin β1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

karyopherin β1 Antibody (H-7): sc-137016

karyopherin β1 Antibody (H-7) is rated 4.6 out of 5 by 11.
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Datasheets

    • karyopherin β1 Antibody (H-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of karyopherin β1 of human origin
    • karyopherin beta 1 Antibody (H-7) is recommended for detection of karyopherin β1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • karyopherin beta 1 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin β1 (H-7): sc-137016.
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I am using karyopherin β1 (H-7): sc-137016 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody Gives very strong and specific signal on Western blots (used at 1:500), but didn't work for me for IF staining (used at 1:250). I tested the antibody on HeLa cells.
Date published: 2018-03-15
Rated 4 out of 5 by from Good for WB This karyopherin β1 Antibody (H-7), sc-137016, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from very good antibody We tested on Hela cell lysate, got very clean and specific band.
Date published: 2017-10-31
Rated 4 out of 5 by from Good for WB it works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from very good function! I use it for IF and WB . It work well in WB at 1:1000 and in IF at 1:200. The band and flouresence is clear and specificity!
Date published: 2017-09-06
Rated 5 out of 5 by from the bind is so clear It's more beautiful than what the instruction shows us.The position is nearly 97kd.
Date published: 2017-07-22
Rated 5 out of 5 by from Interesting why CAN'T I add to the picture on review?The band is so clean,I have wanted to show you ,I use it for VSMC
Date published: 2017-03-16
Rated 5 out of 5 by from Great Antibody for WB, IHC, and IF karyopherin β1 (H-7) works great in all its recommended applications. Strong Western (WB) band at 97 kDa.
Date published: 2017-02-03
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