Anti-JNK1 Antibody (G-4) recommended for detection of JNK1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-JNK1 Antibody (G-4): sc-398989

Anti-JNK1 Antibody (G-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • JNK1 Antibody (G-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 358-397 mapping near the C-terminus of JNK1 of human origin
    • recommended for detection of JNK1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See JNK (D-2): sc-7345 for JNK1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for JNK1 Antibody (G-4) for WB applications. These reagents are now offered in bundles with JNK1 Antibody (G-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with JNK1 (G-4): sc-398989 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good nuclear staining by IF with methanol Good nuclear staining by IF with methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Clean blot with strong band at expected size Clean blot with strong band at expected size in HeLa (A), A-431 (B), SK-N-MC (C), Jurkat (D) and PC-12 (E) whole cell lysates. -SCBT QC
Date published: 2013-05-09
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